摘要
采用稀酸水解和酶水解可将半纤维素和纤维素转化为可发酵性糖 ,再采用基因工程细菌将木质纤维素中的糖转化成乙醇 ,用作燃料 ,是未来能源的补充 ,有利于环保。试验研究表明 ,半纤维素衍生糖补充一定的营养成分 ,经过48h发酵 ,乙醇浓度可达40g/L,是理论产率的90%以上。蔗渣经磨碎、纤维素酶水解 ,蔗渣含量160g/L,经过160h发酵 ,乙醇浓度可达39g/L,加热到45℃维持24h ,然后冷却、接种、发酵 ,乙醇产量可提高50% ;废纸100g/L(干重)的浆液经纤维素酶处理 ,接种K.oxytocaP2发酵72h,可得乙醇40g/L,为理论产率的83%。目前研究出的基因工程细菌有E.coliKO11、K.oxytocaP2和E.chrysanthemi,具有将二元糖和三元糖转化为乙醇的能力 ,接近理论转化率的90% 。
Hemicellulose and cellulose could be changed into fermentable sugar by lumequeic acid hydrolysis and enzyme hydrolysis. Then the genetic engineering bacteria could change the sugar in lignocellulose into alcohol which may be used as fuel and the way was regarded as a resource supply in the future helpful for environmental protection. The research results showed that the alcoholicity of derivative sugar from hemicellulose would be 40g/L (over 90% of the theoretical output)after 48 hours′ fermentation with addition of some nutritional component.The alcoholicity would be 39g/L with bagasse (its content 160g/L) as material,after crash, cellulose enzyme hydrolysis and 160 hours′ fermentation, and when it underwent heating to 45℃ for 24 hours, then cooling, inoculation and fermentation, its alcohol output would increase 50%; and serum of waste paper 100g/L (net weight) would produce 40g/L alcohol (83% of the theoretical output) after treatment with cellulose enzyme and inoculation with K.oxytoca P2 and fermentation for 72h. At present, the bacteria invented by genetic engineering methods are E.coli KO11, K.oxytoca P2 and E.chrysanthemi which are capable of changing binary sugar and ternary sugar into alcohol (90% of the theoretical change rate) and fermenting quarternary stachyose.(Tran.by YUE Yang)
出处
《酿酒科技》
2001年第2期39-42,共4页
Liquor-Making Science & Technology
关键词
酒精
遗传工程
细菌
木质纤维素
预处理
alcohol
genetic engineering
bacterial
lignocellulose
pretreatment
