摘要
目的 :观察电针对实验性血管性痴呆 (VD)大鼠海马神经胶质细胞和毛细血管的影响。方法 :实验用 4 血管阻断模型 ,用Morris水迷宫测定大鼠学习记忆能力 ,并用电镜观察鼠脑海马神经胶质细胞和毛细血管的超微结构变化。结果 :模型组海马神经组织超微结构的形态损害明显 ,胶质细胞变性、水肿 ,毛细血管内皮细胞受损。电针能明显减轻或抑制缺血的大鼠海马神经胶质细胞及毛细血管的损害。结论 :电针明显改善痴呆大鼠的学习记忆能力 ,与其对脑组织神经元。
Objective: To observe the effect of electroacupuncture (EA) on neuroglial cells an d capillaries of hippocampus in vascular dementia (VD) rats. Methods: Rat VD mod el was established by using four-vessel occlusion (4-VO) method, and the rats were randomized into model group, EA group and sham-operation group, with 5 rat s in each group. EA (150 pulses/min, 2.0 mA, 20 min) was delivered to 'Baihui'( GV 20) and 'Dazhui'(GV 14) once daily, continuously for 10 days. The brain tiss ue was cut into sections for observing ultrastructural changes of neuroglial cel ls and capillaries in the hippocampus under transmission electron microscope. Results: In model group, the cellular membrane and the nuclear membrane of neuroglial c ells in hippocampus were not clear, the chromatin condensed, the perinuclear org anelles decreased in number, disintegrated or disappeared, presenting a big blan k region. The hippocampal capillaries showed deformed lumen, indistinct basement membrane, swelling of the endothelial cells, and decrease of nerve synapses. Wh ile in acupuncture group, the ultrastructural changes of neuroglial cells were m uch lighter, such as light swelling of the cellular nuclei and membrane and cyto lipin with condensation of the nuclear chromatin but without blank zone; and the mitochondria were close to normal. The capillaries showed distinct and smooth l umen, basically intact basement membrane with slight swelling at the periphery p art of the membrane, being apparently lighter than those of model group in the s tructural injury. Conclusion: EA could markedly suppress ultrastructural lesion of neuroglial cel ls and capillaries in hippocampus of VD rats, and lessen the damage of cerebral tissue.
出处
《针刺研究》
CAS
CSCD
2003年第4期251-254,共4页
Acupuncture Research
基金
教育部高等院校骨干教师资助计划项目
