摘要
M胆硷受体上行调节具有以下特点:(1)用100nmol/L的人工合成的糖皮质激素地塞米松(dexamethasone,DEX)孵育细胞48h,细胞中M受体量从726.0±25.9增加至1092.0±39.0fmol/10~6细胞,DEX不影响M受体与配基结合的亲和力.(2)DEX的调节作用在一定范围内依赖于时间和浓度,10^(-8)mol/L的DEX达最大效应.(3)DEX可使细胞对标记前体向DNA、RNA和蛋白质的参入量分别增加80.8±5.1%,29.8±3.3%和20.0±3.2%,表明DEX对M胆硷受体的调节作用与促进DNA复制、RNA砖录和受体蛋白质的合成过程有关.
Treatment of CCL137 cells with 100 nmol/L dexamethasone(DEX) for 48 hours caused increase in the number of muscarinic acetylcholine receptor (mAChR) from 726.0±25.9 to 1092.0±39.0 fmol/10~6 cells,without affecting its affinity to ligand.The effect of DEX on mAChR was dose-and time-dependent.The maximum response was induced by 10^(-8)mol/L of DEX,The incorporation of tritiated-thymidine,uridine and leucine into DNA,RNA and protein of the cells was increased by DEX to 80.8±5.1,29.8±3.3 and 20.0±3.2% respectively as compared to the control's.This means that the effect of DEX on mAChR may be due to the enhancement of DNA replication,RNA transcription and receptor protein synthesis.
出处
《军事医学科学院院刊》
CSCD
北大核心
1989年第6期413-417,共5页
Bulletin of the Academy of Military Medical Sciences
关键词
地塞米松
乙酰胆硷受体
上行调节
muscarinic acetylcholine receptor
dexamethasone
up-regulation
