摘要
作者研制了适用于在自动生化仪上测定血清LDH活性的LD-P紫外监测法试剂盒.实验室研究证明,自制试剂盒在Impact 400型自动生化仪上,与西德BM公司,美国Gilford公司同类试剂盒对比测定结果一致,呈非常显著相关.酶促反应的延迟时间短(<20s),血清LDH活性测定线性范围宽,可达1000u/L(37℃),批内变异系数1.1～4.7%.在4～8℃条件下贮存,本试剂盒可稳定7个月,重溶试剂可稳定7天.81名健康成人血清LDH活性测定结果为318.3±61.9u/L(37℃).
The preparation of a kinetic LD-P kit for determination of serum lactate dehydrogenase (LDH) activity with automated analyzer was studied,based on the direct NADH-coupled assay employing pyruvate as the substrate.With the Impact 400 analyzer a comparison test was made among our kit and those purchased from Boehringer Mannheim (W Germany) and Gilford (USA),and a significant correlation among the results was obtained.The findings also proved that our kit is good in precision,linearity and stability.
Serum LDH activity for healthy adults,determined with our kit,is 318.3±61.9u/L (?SD) at 37℃(n=81).
出处
《军事医学科学院院刊》
CSCD
北大核心
1989年第6期450-454,共5页
Bulletin of the Academy of Military Medical Sciences
基金
军事医学科学院科学基金(科基字022号)
关键词
乳酸脱氢酶
紫外监测法
试剂盒
lactate dehydrogenase
reagent kit
automated clinical chemistry analyzer
UV-monitoring method
