摘要
目的 :建立测定人血浆中米氮平浓度的高效液相色谱法。方法 :以美国迪马公司钻石C1 8反相柱 (2 50mm×4 .6mm ,5μm)为色谱柱 ,流动相为甲醇 水 (90∶1 0 ) ,流速为 0 .8mL·min- 1 ,检测波长 2 94nm ,以乙酸乙酯为提取剂。结果 :米氮平高、中、低 (50 0 .0 ,2 50 .0 ,50 .0 μg·L- 1 ) 3个浓度的平均回收率分别为 1 0 0 .2 1 % ,98.47% ,97.0 0 % ,日内、日间差RSD均低于 6 % ;分析方法的定量测定下限为 1 5 .0 μg·L- 1 。线性范围为 2 5 .0～ 1 0 0 0 .0 μg·L- 1 。结论 :该方法灵敏、准确、简单、快速 ,可用于临床血浓监测和药动学研究。
OBJECTIVE To develop an HPLC method for quantitative determination of mirtazapine in blood plasma.METHODS Blood plasma samples were extracted with hexane to remove lipid and interfering substances, then extracted with ethyl acetate. The residue was analyzed with a reverse phase HPLC system [C 18 column: 4.6 mm × 250 mm , 5 μm ; Mobile phase:MeOH-H 2O (90:10,V/V); UV detection: 294 nm ].RESULTS The average recoveries for mirtzapine hydrochloride were 100.21% , 98.47% and 97.00% ,respectively. The within-day and day-to-day relative standard deviations were lower than 6%. The calibration curves had good linearity ( r = 0.999 ) within a concentration range of 25.0 ～ 1000.0 μg ·L -1 . The limit of quantitation for mirtazapine was 15.0 μg ·L -1 .CONCLUSIONS The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of mirtazapine blood plasma and its phamacokinetic studies.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第2期78-80,共3页
Chinese Journal of Hospital Pharmacy
