摘要
Objective: To study the proliferation, migration and metaplasm of residual rabbit lens epithelial cells (LECs) after extracapsular cataract extraction(ECCE)based on the rabbit capsular bag model in vitro. Methods: Sham cataract surgery, including anterior capsulorhexis, nucleus hydroexpression and aspiration of lens fibers, was performed on 20 rabbit lens. The capsular bags were isolated and pinned to sterile non-toxic silicone rings on petri dishes. The capsular bags were incubated with Eagle's minimum essential medium (DMEM) supplemented with 10% fetal calf serum (FCS) and monitored for 3 weeks by phase-contrast microscopy, after which light microscopy was performed on them.Results: After a latent period of 2-3 d, outgrowth was observed across the posterior capsule. Growth proceeded rapidly so that the posterior capsule was totally covered by a confluent monolayer of cell at 6-8 day. Capsular wrinkles became increasingly apparent as time progressed, causing a marked rise in light scatter. An increase in capsular tension also came.Conclusion: This model exhibits many of the in vito characteristics of the lens capsule after extracapsular surgery and may prove useful in further elucidating the cellular mechanisms of posterior capsule opacification and developing strategies for inhibiting cell growth with this system.
目的 :建立一种囊袋模型 ,研究兔晶体囊外摘除术 (ECCE)后残余的晶体上皮细胞 (LECs)在体外增殖 ,移行 ,化生的情况。方法 :2 0只兔眼 ,体外模拟白内障手术 ,并游离晶体囊袋 ,固定于硅胶环上 ,置含 10 %胎牛血清的DMEM营养液中培养 3周。分别用相差显微镜和光镜观察不同培养时期后囊上LECs的生长情况。结果 :经过 2～ 3天的潜伏期 ,后囊周边部开始出现LECs ,细胞增殖速度较快 ,约 6～ 8天可完全覆盖后囊。组织学可见均质红染的后囊膜上被覆一层或多层排列紧密 核深染胞浆丰富的LECs。结论 :应用这种囊袋模型 ,进行LECs体外培养 ,较为真实的表现了体内ECCE术后的多种变化 ,有助于更深一步探讨后囊膜混浊的发生机制 ,并为筛选防治后发性白内障的有效药物 。
基金
SupportedbytheDevelopmentFoundationofNanjingMedicalUniversit
