摘要
目的 了解脂多糖 (L PS)对肺、肝、脾组织中与 L PS信号转导有关的受体的基因表达情况的影响。方法 L PS刺激前后取小鼠肺、肝、脾组织总 RNA,采用逆转录聚合酶链反应 (RT PCR)半定量测定脂多糖结合蛋白 (L BP)、CD1 4、Toll样受体 2 (TL R2 )、TL R4和肿瘤坏死因子 α(TNFα)等基因的表达情况。结果 正常组织中 ,肺有 TL R2的表达 ,肝有 TL R2、CD1 4、L BP的表达 ,脾有 TL R2的表达 ;注射 L PS后的 5 h内 ,肺组织 TL R2、TL R4、CD1 4和 TNFα,肝组织 TL R2、CD1 4、L PB和 TNFα,脾组织 TL R2、TL R4、CD1 4和TNFα的基因表达均呈逐渐增加趋势。结论 正常情况下 TL R2等基因在不同组织中的表达有差异 ,L PS刺激 5 h内各基因的表达提高 ,从而提高机体对 L PS等病原体模式识别分子的反应性。
Objective To investigate the gene expression of some lipopolysaccharide(LPS) receptors after LPS stimulation. Methods The total RNA from normal and LPS-challenged mice was extracted by Trizol reagent and the gene expression of Toll-like receptor 2(TLR2), TLR4, CD 14, LPS-binding protein(LBP) and tumor necrosis factor-α(TNF-α) were measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). Results The gene of TLR2 was expressed in normal lungs and spleen tissues, and TLR2, CD 14, LBP in liver. After challenged by LPS, the expressions of TLR2, TLR4, and TNF-α in lungs, TLR2, CD 14, LBP and TNF-α in liver, TLR2, TLR4, CD 14, and TNF-α in spleen were increased at 1, 3, and 5 hours. Conclusion LPS might alert the ability against pathogen-associated molecules by inducing or enhancing the expression of genes that involved in the LPS signal transduction.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第2期73-76,T001,共5页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目 (3 0 2 7165 1)
