摘要
珍宝丸Ⅱ与珍宝丸Ⅲ均为蒙药 ,其中珍宝丸Ⅱ由人工合成物制成 ,珍宝丸Ⅲ由天然药物制成。文章以甲醇作为提取溶剂 ,采用索氏提取器提取蒙药珍宝丸Ⅱ ,Ⅲ中黄酮类化合物 ,用比色法测定其含量 ,珍宝丸Ⅱ作为供试品对该实验方法进行考察 ,结果表明 :定量测定线性范围为 8 0 5～ 4 8 2 8μg·mg- 1 ,加样回收率为 99 4 9%～ 10 0 5 0 % ,RSD为 0 5 4 % (n =3)。珍宝丸Ⅱ中黄酮类化合物的含量范围在 1 4 7～ 1 5 5mg·g- 1 之间 ,珍宝丸Ⅲ中黄酮类化合物含量范围在 2 80～ 3 0 0mg·g- 1 之间。该法操作简便 ,经济可靠 ,重现性好 ,便于推广使用 ,适合于各种丸剂药物中黄酮类化合物含量的测定。
Both Zhenbao pills Ⅱ and Ⅲ are Mongolian medicine of a kind. Zhenbao pill Ⅱ was the artificial syntheses and Zhenbao pill Ⅲ was made from natural materials. In this paper, the flavonoids of Mongolian medicine Zhenbao pills Ⅱ and Ⅲ were extracted by Soxhlet apparatus with methyl alcohol as extracting agent, the colorimetric method was applied to the determination of flavonoids, and the experimental procedure was studied with Zhenbao pill Ⅱ as the test sample. The result showed that the linear range of quantitative determination was 8.05-48.28 μg·mg -1. The standard addition recovery(SAR) was 99.49%-100.50%. The RSD(n=3) was 0.54%.The range of contents of flavonoids was 1.47-1.55 mg·g -1 in Zhenbao pill Ⅱ and was 2.88-3.00 mg·g -1 in Zhenbao pill Ⅲ. This method was simple and accurate with good reproducibility, and is suitable for the determination of flavonoids in all kinds of pills. The contents of flavonoids can be used to prove whether Zhenbao pill is artificial syntheses or natural material.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第1期89-91,共3页
Spectroscopy and Spectral Analysis
基金
内蒙古自治区教育厅科研基金
