摘要
采用电喷雾多级串联质谱对苦参中黄酮类化合物二氢黄酮及二氢黄酮醇类化合物的特征质谱行为进行了研究 .实验结果表明 ,两类化合物在电喷雾多级串联质谱条件下均可以在 C环发生开环断裂 ,但断裂的位点不同 ;两类化合物生成的碎片离子也有很大差异 ,提出了由二氢黄酮醇类化合物 C环上 3位连接的—
Two kinds of flavonoids from Sophora Flavecens Aits, dihydroflavonoids and dihydroflavonols, were studied by using electrospray ionization tandem mass spectrometry(ESI-MS n). [M-H] - of these two kinds of flavonoids were observed in the negative ion mode under ESI conditions, which provided us the molecular weights of them. Cross-ring reactions, arisen from MS 2 spectra of both dihydroflavonoids and dihydroflavonols, gave the fragment ions by the loss of neutral species. The differences of their cleavage positions were then proposed, dihydroflavonoids cleave at C2—O bond and C4—C10 bond, but dihydroflavonols cleave at C2—O bond and C3—C4 bond. The loss of neutral species from dihydroflavonoids and dihydroflavonols were also discussed, the former were several relative big neutral species, while the latter were some small species, such as CO, CO_2, H_2O and B-ring. The differences between dihydroflavonoids and dihydroflavonols were mainly arisen by hydroxy group at C-3 of the C-ring, which were the characteristic for dihydroflavonoids and dihydroflavonols under the ESI-MS n conditions.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第2期284-288,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 173 0 5 7)资助
