摘要
利用透射电镜对感染玉米矮花叶病毒山东分离物 (MDMV SD)的不同抗性玉米叶片细胞超微结构进行了观察。结果表明 ,与健康植株相比 ,无论是在抗病品种鲁玉 16还是在感病的自交系鲁原 92细胞中 ,MDMV SD与玉米植株之间的互作都可以导致寄主发生明显的细胞病理学变化。但是 ,在抗病和感病植株中叶片细胞超微结构的变化存在明显差异。在抗病植株细胞中 ,虽然叶绿体、线粒体等主要细胞器的结构损伤较轻 ,但线粒体和过氧化物酶体的数量明显增多 ,细胞质中有风轮体、束状体和片层聚集体 3种内含体 ,存在于细胞质中的病毒粒子相对较少 ,胞间连丝数量少或几乎观察不到 ,未观察到细胞膜结构增生现象 ;在感病植株细胞中 ,叶绿体、线粒体损伤严重 ,叶绿体的类囊体肿胀 ,外膜破裂 ,细胞质内病毒粒子相对较多 ,并含有 4种内含体 ,即风轮体、束状体、片层聚集体和高电子密度体 ,细胞壁上的胞间连丝数量明显增多 ,受侵染细胞大量产生多泡体 。
Ultrastructural alterations in foliar cells were studied in leaves of resistant maize variety Luyu16 and susceptible maize inbred Luyuan92 infected by maize dwarf mosaic virus Shandong isolate (MDMV-SD), respectively. The results showed that marked cytopathological alterations were observed both in resistant and susceptible plants while compared with that in healthy plants. However, some ultrastructural alterations, which observed in resistant plants, were different from those in susceptible plants. In resistant plants, which infected by the virus, the main organelles, including chloroplasts and mitochondria, were slightly destroyed, the amount of mitochondria and peroxisome was increased. A few or no plasmodesmata were observed. There were three kinds of inclusions including pinwheel, bundle and laminated aggregate, and the virus particles in the cytoplasm. In susceptible plants, which infected by the virus, the chloroplasts were heavily disrupted, including thylakoid swelling and envelope broking. The virus particles were more than those in the resistant variety. Four kinds of inclusions including pinwheel, bundle, laminated aggregate and high electon-dense body appeared in cytoplasm. Plasmodesmata and plasma membrane were abundant, and there were frequent invaginations of the plasma membrane that led to the formation of vesicles and myelin-like structures.
出处
《中国农业科学》
CAS
CSCD
北大核心
2004年第1期72-75,T001,T002,共6页
Scientia Agricultura Sinica
基金
中国博士后科学基金资助项目 ( 2 0 0 2 0 3 1168)
山东省优秀中青年科学家科研奖励基金资助项目 ( 0 2BS0 2 5 )
山东农业大学博士后基金资助项目 ( 760 3 6)
关键词
玉米
矮花叶病毒
叶片细胞
超微结构
病理变化
Maize dwarf mosaic virus
Foliar cell
Ultrastructure
Cytopathological alteration
