摘要
通过测定超声处理前后发酵液中核酸、蛋白质及1,6-二磷酸果糖(FDP)浓度的变化,研究了功率超声及有机溶剂对酵母细胞膜通透性的影响.结果表明随着超声作用时间的增加,功率为600、500、400W的处理都使胞内核酸、蛋白质及FDP的渗出浓度逐渐提高,其中,600W超声的作用最为明显.超声处理后,细胞的存活率都逐渐减小,即细胞的破碎程度增大.其中,随着超声作用时间的延长,600W的超声使细胞存活率下降最快,500W超声次之,400W超声使细胞的存活率下降最慢.有机溶剂中,甲苯和氯仿对胞内物质的渗出率影响非常大,但甲苯和氯仿对细胞的损伤非常严重.乙醚对细胞的存活率影响不大.
In this project, the effect of ultrasounding treatment with three sorts of organic solvents on yeast cell membrane permeability was studied, by measuring the concentration of nucleic acid, protein and fructose-1,6-diphosphate (FDP) before and after the treatment. The result showed that by increasing the effective time, the concentration of nucleic acid , protein and FDP in the broth increased gradually after ultrasound treatment with power of 600, 500 and 400 W, the most notable effect being achieved by 600 W ultrasounding. After treatment with those ultrasounds, the livability of cells decreased. That was to say, the number of disintegrated cells increased. With the extension of effective time, livability decreased most quickly when applying 600 W, then 500 W and 400 W. Among organic solvents, toluol and chloroform affected exudation from the cell very much, but damage the cell severely. Aether has little influence on the cell livability.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2004年第1期74-76,共3页
Journal of South China Agricultural University
基金
国家自然科学基金资助项目(10174021)
