摘要
目的 研究结核性腹膜炎患者外周血及腹水T淋巴细胞亚群的变化 ,探讨其全身及局部免疫功能变化。方法 流式细胞仪测定 3 8例结核性腹膜炎治疗前、治疗后及 2 9例肝硬化并腹水患者、15例正常人的外周血和 (或 )腹水T淋巴细胞亚群。结果 ①结核性腹膜炎患者治疗前外周血T淋巴细胞 (CD3 + )、T辅助 /诱导淋巴细胞亚群 (CD4+ )、T辅助淋巴细胞 /T抑制淋巴细胞亚群 (CD4+ /CD8+ )较正常对照明显降低 (P <0 0 1) ,抗结核治疗后较治疗前升高 (P <0 0 1) ;②结核性腹膜炎患者腹水CD3 + 、CD4+ 、CD8+ 、CD4+ /CD8+ 显著高于肝硬化患者 (P <0 0 1) ,与外周血比较除CD8+ 降低 ,其余亦明显升高 (P <0 0 1)。结论 结核性腹膜炎患者外周血及腹水T淋巴细胞亚群存在明显异常 ,其免疫功能紊乱 ,表现为全身细胞免疫力低下而腹膜腔局部免疫力增强 。
Objective To investigate changes of T lymphocyte subsets in peripheral blood and ascitic fluid in patients with tuberculous peritonitis and explore changes of systemic and local immunological function of patients.Methods The levels of T lymphocyte subsets in peripheral blood and(or) ascitic fluid were detected by flow cytometer in 38 cases of tuberculous peritonitis before and after therapy, 29 cases of cirrhosis of liver complicated with ascites formation and 15 cases of normal subjects as control group.Results ①The levels of T lymphocyte(CD 3 +), helper/inducer T lymphocyte(CD 4 +)and helper T lymphocyte /suppressor T lymphocyte(CD 4 +/CD 8 +)were obviously decreased in patients of tuberculous peritonitis,as compared with normal subjects(P<0 01).All parameters were increased in the patients treated with anti-tuberculosis drug(P<0 01).②The levels of CD 3 +, CD 4 +, CD 8 + and CD 4 +/CD 8 in ascitic fluid in patients of tuberculous peritonitis were significantly higher than those in patients with cirrhosis of liver (P<0 01)and they obviously increased compared with those in peripheral blood except that CD 8 + descreased (P<0 01).Conclusions The levels of T lymphocyte subsets in peripheral blood and ascitic fluid in patients of tuberculous peritonitis are obviously abnormal. The patients exist immunological function disorder,showing the low systemic cellular immunity and high local immunity of peritoneal cavity. The detection of T lymphocyte subpopulation may be a new diagnostic index for tuberculous peritonitis.
出处
《中国医师杂志》
CAS
2003年第7期919-921,共3页
Journal of Chinese Physician
