期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

内皮素-1受体在兔不全梗阻性膀胱中的研究

Studies on endothelin-1 receptor in a rabbit partial bladder outlet obstruction
在线阅读 下载PDF
导出
摘要 目的 :研究内皮素 1受体 (ET 1R)在兔不全梗阻性膀胱中的变化和意义。　方法 :采用受体的放射配基结合分析技术 (RBA) ,对 10只成年雄性新西兰白兔不全梗阻性膀胱 (兔不全梗阻性膀胱模型 ,梗阻 6周为实验组 )及 10只同龄雄性新西兰白兔无梗阻性膀胱术后 6周 (对照组 )的膀胱体及膀胱颈部平滑肌组织ET 1R进行研究。结果 :在膀胱体平滑肌组织中ET 1R结合量 ,实验组较对照组明显增多 (P <0 .0 2 ) ,而膀胱颈平滑肌组织中ET 1R结合量无明显变化 (P >0 .2 )。实验组较对照组的膀胱重量明显增加 (P <0 .0 0 1)。　结论 :兔不全梗阻性膀胱的膀胱体平滑肌组织中ET 1R结合量明显增多。ET 1具有收缩平滑肌和促平滑肌、纤维结缔组织增生 (有丝分裂 )的作用 ,故ET 1R的上调是导致兔不全梗阻性膀胱壁结构改变及功能紊乱的重要因素之一。 Objectives : To study endothelin 1 receptor in a rabbit model partial bladder outlet obstruction(BOO). Methods :We used the rabbit model of partial bladder outlet obstruction to study endothelin 1 receptor(ET 1R) with radioligand binding assay of receptors (RBA).Partial bladder outlet obstruction was induced in adult male New Zealand white rabbits(ten rabbits as experiment group,time of obstruction for six weeks).Sham operated age matched rabbits acted as control(ten rabbits as control group,time of postoperation for six weeks). Results: The endothelin 1 binding sites were significantly increased in the BOO detrusor smooth muscle( P <0.02).However, there was no changes in the BOO bladder neck . BOO bladder weights were significantly increased( P <0.001). Conclusions: The endothelin 1 binding sites were significantly increased in the BOO detrusor smooth muscle . Endothelin 1 has mitogenic and potent contractile properties for smooth muscle cells,has mitogenic property for connective tissue.This suggests the up regulation of ET 1R is associated with altered bladder structure and disfunction of rabbit partial bladder outlet obstruction .
作者 程文 周四维 高建平 张征宇 葛京平
机构地区 南京军区南京总医院泌尿外科 华中科技大学同济医学院附属同济医院泌尿外科
出处 《医学研究生学报》 CAS 2003年第10期730-732,共3页 Journal of Medical Postgraduates
基金 中国博士后科学基金资助课题 (批准号 :2 0 0 2 0 3 2 189)
关键词 内皮素-1受体 不全梗阻性膀胱 实验兔 Endothelin 1 receptor Rabbit partial bladder outlet obstruction Experiment rabbit
分类号 Q95-33 [生物学—动物学]
  • 相关文献

参考文献12

  • 1Buttyan R,Chen MW,Lein RM. Animal models of bladder outlet obstruction and moleculr insights into the basis for the development of bladder dysfunction [J]. Eur Urol, 1997, 32 (Suppl 1):32-39.
  • 2Tejada I, Mueller JD, de Las Morenas A et al . Endothelin in the urinary bladder 1. Synthesis of endothelin-1 by epithelia,muscle and fibroblasts suggests autocrine and paracrine cellular regulation [J]. J Urol, 1992, 148(4):1290-1298.
  • 3Maggi CA,Giluliani S,Patacchii R et al. A potent contractile activity of endothelin on the human isolated bladder [J]. Br J Pharmacol, 1989, 96(4):755-777.
  • 4Warner TD, Allock Gh,Mickley EJ et al. Comparative studies with the endothelin reeptor antagonist BQ123 and PD142893 indicates at least three endothelin receptors[J]. J Cardiovasc Pharmacol, 1993 ,22 (Suppl 8):S117-120.
  • 5Burnett AL.Nitric oxide control of lower genitourinary tract functions: A review[J]. Uology , 1995, 45(6):1071-1083.
  • 6Persson K. Nitric oxide and relaxtion of pig lower urinary tract [J]. B J pharmacol, 1992, 106(2): 416-422.
  • 7Hirata Y,Emori T,Eguchi S et al. Endothelin receptor subtype B mediates synthesis of nitric oxide by cultured bovine endothelial cells [J]. J Clin Invest ,1993, 91(4):1367-1373.
  • 8Goligorsky MS,Tsukahara H,Magazine H et al. Termination of endothelin signalling:role of nitric oxide[J]. J Cell Physiol ,1994, 158(3):485-494.
  • 9程文,周四维,高建平,张征宇,葛京平,周文泉,马宏青,周水根,魏武,薛松,梁培禾,李汉平,蔡荣星.血浆内皮素-1在兔不全梗阻性膀胱中的研究[J].医学研究生学报,2003,16(4):266-268. 被引量：7
  • 10Wada Y, Latifpour J,Sanematsu H et al. Age-related changes in contracticle responses of rabbit lower urinary tract to endothelin[J]. J Urol ,2000, 164(3pt 1):806-813.

二级参考文献12

  • 1Robert ML, Janice H, Alan J. The effect of short-term obstruction on urinary bladder function in the rabbit[J]. J Urol,1984,132:789-791.
  • 2Khan MR, Dashwood CS, Thompson FH, et al. Up-regulation of Endothelin-B receptor(ETB) and ETB receptor-mediated rabbit detrusor contraction in partial bladder outlet obstruction[J].BJU Int,1999, 84.714-719.
  • 3Tejada I, Mueller JD, de Las Morenas A, et al. Endothelin in the urinary bladder 1. Synthesis of endothelin-1 by epithelia、muscle and fibroblasts suggests autocrine and paracrine cellular regulation[J]. J Urol, 1992, 148(4):1290-1298.
  • 4Traish A,Moran E, Krene RJ, et al. Endothelin in the urinary bladder: II Characterization of endothelin receptor subtypes[J]. J Urol,1992, 148(4):1299-1306.
  • 5Yanagisawa M, Kurihara H,Kimura S,et al.A novel potent vasoconstrictor peptide produced by vascular endothelial cells[J].Nature,1988,332:411.
  • 6Inoue A,Yanagisawa M,Kimura S,et al. The human endothelin family:Three structurally and pharmacologically distinct isopeptides predicted by three separate genes[J].Proc Natl Acad Sci USA, 1989,86:2863-2867.
  • 7Imai T,Hirata Y, Emori T,et al. Induction endothelin-1 gene by angiotansin and vasopressin in endothelial cells[J]. Hypertension, 1992, 19(6pt 2):753-757.
  • 8Le Monner de Gouville AC,Lippton HL, Cavera I, et al. Endothelin-a new family of endothelium-derived peptides with wide spread bioligical properties[J]. Life Sci, 1989, 45(17):1499-1513.
  • 9Langenstroer P, Tang R,Divrsh B, et al. Endothelins in caninegenitourinary tissue[J]. J Urol, 1997, 157(3):1044-1048.
  • 10Lecci A,Giuliani S,Santicioli P, et al. Intracerebroventricular administration of endothelins:effects on the supraspinal micturition reflex and blood pressure in the anaesthetized rat[J]. Eur J Pharmacol, 1991, 199(2):201-207.

共引文献6

医学研究生学报
2003年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部