摘要
目的:建立胃癌细胞系H.pylori感染动物转移体外模型,初步探讨H.pylori感染在胃癌转移过程中的可能作用及其机制.方法:将H.pylori活菌与胃癌细胞系BGC-823低转移细胞株C8细胞共培养后,行裸鼠接种,观察H.pylori感染组与未感染组移植瘤生长情况及裸鼠肝、肺转移情况,免疫组化检测两组移植瘤中MMP-2,MMP-3,TIMP-2及TIMP-3蛋白的表达.结果:H.pylori感染组移植瘤重量与未感染组比较有差异(P<0.05),病理检查示感染组2个肺转移,而未感染组无转移.免疫组化结果示感染组MMP-2,MMP-3,TIMP-2,TIMP-3蛋白的表达阳性率分别为:77.8±9.6%,64.5±6.9%,57.6±12.2%,40.0±9.2%;而未感染组分别为61.2±9.7%,53.1±5.8%,54.3±10.9%,53.0±6.6%,二者比较除TIMP-3蛋白的表达无显著性差异外,MMP-2、MMP-3、TIMP-2蛋白的表达均有显著性差异(P<0.05).结论:H.pylori感染能促使裸鼠移植瘤的生长及肺转移,建立了H.pylori感染与胃癌的动物转移体外模型;H.pylori感染可能通过促使转移正相关因子MMP-2、MMP-3表达增高,转移负相关因子TIMP-2蛋白表达降低,而在胃癌转移过程中发挥重要作用.
AIM:To establish an animal model to study the effect of H. pylori on metastasis, and to explore the possible mechanism and the relationship between H. pylori and gastric cancer metastasis. METHODS:Using BGC-823 cell cocultured with H .pylori, the growth of tumors and metastasis were observed in 8- week nude mice after the first intraumoral injection of the group of H.pylori infection and the group of no infection. The expression of MMP-2, MMP-3, TIMP-2 and TIMP-3 were investigated by immunohistochemical staining. RESULTS:There was a significant difference in the weight of tumors between the group of H.pylori infection and the group without H.pylori infection in 8-week nude mice after the first intratumoral injection (P <0.05). There were two lung metastases in group of H .pylori infection. The positive rates of MMP-2, MMP-3, TIMP-2 and TIMP-3 in the group of H. pylori infection by immunohischemical staining were 77.8±9.6 %, 64.5±6.9 %, 57.6±12.2 % and 40.0±9.2 %, and 61.2±9.7 %, 53.1±5.8 %, 54.3±10.9 % and 53.0±6.6 % in the group without H.pylori infection, respectively. The expression of MMP-2, MMP-3 and TIMP-2 were significantly higher in the group of H. pylori infection than those in the group without H.pylori infection (P <0.05), but the expression ofTIMP-3 has no significantly change. CONCLUSION:H.pylori infection can accelerate the tumor growth in nude mouse and lung metastasis. The expression of MMP-2 and MMP-3 increases and TIMP-2 decreases in H .pylori infection, suggesting H .pylori may enhance gastric cancer metastasis by regulating the expression of metastasis- correlated factors.
出处
《世界华人消化杂志》
CAS
2003年第5期544-546,共3页
World Chinese Journal of Digestology
基金
湖南省科技厅资助项目
No.01SSY2008-9
湖南省卫生厅资助项目
No.2001-Y23/1.0~~
