摘要
目的 探讨中国人缺血性脑卒中与凝血因子、细胞粘附分子、血小板膜糖蛋白、血浆凝血酶原激活物抑制剂 1以及肿瘤坏死因子基因多态性的关系。方法 利用PCR技术和分子杂交技术对112 2例缺血性脑卒中患者进行 10个基因 17个多态性位点的检测和分析 ,并与 112 3例非卒中对照进行比较。结果 缺血性脑卒中患者肿瘤坏死因子 βthr2 6asn多态性分布与对照组相比具有显著差异。其他基因多态性的基因型频率和等位基因频率与对照组相比无明显差异。结论 肿瘤坏死因子βthr2 6asn多态性分布与中国人缺血性脑卒中的发生具有关联性 ,提示TNFβthr2
Objective To determine whether genetic polymorphisms of coagulate factors, cell adhesive molecules, platelet glycoprotein, plasminogen activator inhibitor 1 and tumor necrosis factor are associated with ischemic stroke in Chinese population. $$$$ Methods This study genotyped up to 17 polymorphisms within 10 genes. The polymorphisms of related genes were studied by molecular and genetic techniques. The assay required PCR amplifications per sample, followed by detection of the alleles present with immobilized probes arrayed in a line format on backed nylon membrane strips. $$$$ Results A significant difference in the distribution for TNF β thr26asn polymorphism between these two groups was found. No other genes polymorphisms was found to be associated with ischemic stroke. $$$$ Conclusion The polymorphism of TNF β thr26asn is associated with ischemic stroke in Chinese population.$$$$
出处
《高血压杂志》
CSCD
2003年第2期116-120,共5页
Chinese Journal of Hypertension
