摘要
目的 观察血管紧张素Ⅱ对培养心肌细胞表达TNF α的影响。方法 采用新生大鼠心肌原代培养和免疫组化 ,RT PCR评价用 10 -7M血管紧张素Ⅱ ,10 0ng/ml脂多糖 (LPS)对心肌细胞表达TNF α的影响。结果 TNF α的免疫组化染色在对照心肌细胞呈阴性 ,10 0ng/ml脂多糖和 10 -7M血管紧张素Ⅱ作用心肌细胞 2 4小时、4 8小时后 ,心肌细胞胞浆中出现棕色颗粒 ,随着作用时间的延长有增强趋势。与单纯培养的对照心肌细胞相比 ,10 -7M血管紧张素Ⅱ ,10 0ng/ml脂多糖刺激心肌细胞 2 4小时后心肌细胞TNF α表达的mRNA显著增高 ,刺激 4 8小时 ,TNF αmRNA进一步增高。结论 正常情况下培养的新生大鼠心肌细胞表达的TNF α在蛋白质水平和mRNA水平均极低 ,10 -7M血管紧张素Ⅱ可以促使培养的心肌细胞表达TNF α 。
Objective To study the production of TNF α in cardiac myocytes stimulated by angiotensin Ⅱ Methods In primary cultured rat myocytes, we use 10 -7 M angiotensinⅡ(AngⅡ) or 100 ng/ml lipopolysaccharide (LPS) to stimulate the cardiac myocytes for 24 or 48 hours.\ Using immunohistochemistry to asseess the TNF α in situ. We performed RT PCR to measure TNF α mRNA in myocytes as well. Results TNF α immunohistochemistry staining was negative in control myocytes, but it was positive in myocytes treated with Ang Ⅱ or LPS. With the prolongation of the treating time, there is a tendency that the increasing of stained granule was obvious. The optical dense ratio TNF α PCR product was obviously increased in Ang Ⅱ or LPS treated myocytes in a time dependent manner. Conclusion Both protein and mRNA levels of TNF α were very low in the normal cultured neonatal rat myocytes,they were obviously increased in Ang Ⅱ treated myocytes,suggested TNF α may be an effector of Ang Ⅱ in myocytes.
出处
《高血压杂志》
CSCD
2003年第2期161-164,共4页
Chinese Journal of Hypertension
基金
国家自然科学基金资助项目编号 30 0 0 0 2 2 1
