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HSP90基因在前列腺癌细胞凋亡中的相关研究

Study on the HSP90 Gene During the Apoptosis of Prostate Carcinoma Cell Line DU-145 Induced by Retinoic Acid
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摘要 目的 :克隆全反式维甲酸诱导前列腺癌细胞系DU 145细胞产生凋亡的相关基因。探讨前列腺癌细胞产生凋亡的分子机制。方法 :应用全反式维甲酸诱导前列腺癌DU 145细胞凋亡 ,建立细胞凋亡模型 ,采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。结果 :在前列腺癌DU 145细胞凋亡过程中 ,克隆出包括HSP90基因在内的多个基因序列。结论 :全反式维甲酸诱导的前列腺癌细胞DU 145凋亡有多基因参与其中 。 Objective To clone the apoptosis-related genes induced by all trans-retinoic acid(ATRA) from prostatic carcinoma cell line DU-145,and investigate the molecular mechanism of the apoptotic process.Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.Results Multiple genetic sequences,including HSP90 gene,were cloned during the apoptosis of prostatic carcinoma cell line DU-145.Conclusions ATRA-induced apoptosis of prostatic carcinoma cell is a complexed process with multiple genes involved.HSP90 gene and other apoptosis related genes may play important roles during the process of apoptotic process.
作者 邵晨 李振钢 晏伟 秦卫军 邵国兴
机构地区 第四军医大学西京医院泌尿外科 解放军总医院医疗科 第四军医大学病理学教研室
出处 《肿瘤防治杂志》 2003年第4期396-398,共3页 China Journal of Cancer Prevention and Treatment
关键词 热休克蛋白 前列腺肿瘤 细胞凋亡 基因克隆 HSP90基因 前列腺癌 HSP90 Prostatic carcinoma Apoptosis Clone
分类号 R737.25 [医药卫生—肿瘤]
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肿瘤防治杂志
2003年 第4期

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