摘要
目的 :研究人类珠蛋白基因转录水平的定量表达。方法 :设计 7种珠蛋白基因mRNA的特异性引物 ,采用RT -PCR技术 ,探索相同条件下同时扩增 7种珠蛋白mRNA ,并定量其表达水平。结果 :正常胎儿脐血中同时扩增出 7种珠蛋白基因 ,各片段分子量符合引物设计片段长度 ;K5 6 2细胞株检测有α、Gγ、Aγ、δ、ε、ζmRNA ,无 β -mRNA ;阴性对照B淋巴细胞株XJH未扩增出任一珠蛋白基因mRNA。结论 :应用RT -PCR技术同时扩增 7种珠蛋白基因mRNA的定量方法是可行的 ,具有特异性。
Objective:To study the quantitative expression of human globin genes in transcription levels.Methods:Specific primers of globin mRNA were designed and RT-PCR was applied to amplify seven kinds of globin mRNA (α,β,Aγ,Gγ,ζ,ε,δ)under same condition at one time, then their products were measured with the help of special image software.Results:Seven kinds of globin mRNA were amplified successfully at one time in normal umbilical cord blood,and the length of amplified globin mRNA was in accordance with that of designed; α,Gγ,Aγ,δ,ε,ζ mRNA were detected in K562 cells except β. None of globin mRNA was detected in B lymphocyte XJH which used as negative control.Conclusion:The quantitative method of seven kinds of globin mRNA by RT-PCR technique at one time is practicable and specific.
出处
《西北国防医学杂志》
CAS
2003年第6期407-409,共3页
Medical Journal of National Defending Forces in Northwest China
基金
国家自然科学基金资助项目 ( 3 9770 784)
