摘要
目的优选胃舒颗粒中莪术挥发油的提取及β-环糊精(β-CD)包合工艺。方法采用正交试验设计,以莪术油提取量为指标优选提取工艺;以包合物收率及挥发油包封率为指标优选包合工艺,并采用紫外分光光度法测定挥发油的含量。结果优选提取工艺条件为:莪术药材粉碎成粗颗粒,加12倍量水,蒸馏提取6h;包合物最佳制备工艺为:β-CD与莪术油投料比为8∶1,加2倍量水,研磨时间60min。莪术挥发油的醋酸乙酯溶液的紫外光谱在253nm波长处有最大吸收。结论采用该工艺莪术油提取率高,β-CD包合后挥发油粉末化并使其稳定性增强。
Objective To optimize the conditions of process for extraction of volatile oil of Rhizoma Zedoariae (RZ) in Weishu Granules and preparation of its β - cyclodextrin- inclusion complexes. Methods Orthogonal design was adopted. Extraction content of RZ volatile oil was used as the index for the optimizing of extraction process. The amount of inclusion complexes and efficient inclusion rate served as the indexes for the optimizing of inclusion process. Ultraviolet spectrophotometry was applied to detect the content of volatile oil. Results The optimal extraction conditions were: gringding the crude RZ into coarse grains, adding 12- fold water and distilling the mixture for 6 h; the optimal inclusion conditions were: β - cyclodextrin and volatile oil were mixed in the proportion of 8 :1, 2- fold water was added into the mixture and the mixture was triturated for 60 min. The maximum UV spectrum absorbance of aethylis acetas liquor of valatile oil located at wavelength of 253nm. Conclusion Higher extraction rate of RZ volatile oil can be achieved by this technique; volatile oil is powdered and its stability increased after being included by β - cyclodextrin.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第1期56-58,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
胃舒颗粒
莪术挥发油
Β-环糊精
紫外分光光度法
正交试验
Weishu Granules
Volatile oil of Rhizoma Zedoariae
β - cyclodextrin
Ultraviolet spectrophotometry
Orthogonal test
