摘要
目的:本文用酶联免疫吸附测定(EIA)和鼠疫间接血凝试验(PHA)的方法,对鼠疫病人发病初期血清(分别采于3天和10天)进行了检测。结果:在发病3天,病人的血清中(EIAIgM)滴度为1:1000,而(PHA)阴性,但在发病10天病人血清中能检出(PHA)阴性,滴度为1:40。该试验结果说明鼠疫病人发展初期,未能产生足够的抗体使之出现阴性凝集滴度,阳性凝集需要有一定的抗体浓度。早期循环的FI抗原特异性的IgM分子的出现将应用于临床病人诊断是否为鼠疫感染是非常重要的辅助手段。
This text applied EIA and PHA to detect the earlier period serum of plague patient (adopted respectively in 3th day and 10th day). It is found that the IgM antibody dilution rate which was in 3th day of the disease was 1:1000 by EIA, and negative by PHA. While in 10th day od the disease , the dilution rate was 1:40, and positive by PHA. This experiment makes clear that it is the positive result which was in the earlier period of plague patient could not present. Positive coagulation demands a certain of antibody. The emergence of FI antigen or its particularity IgM antibody in earlier period circulation is count for much as an assistant means to examine plague infection of not.
出处
《医学动物防制》
2003年第12期755-756,共2页
Journal of Medical Pest Control
关键词
IGM抗体
检测
鼠疫
早期诊断
the serum of plague patient
EIA
PHA
diagnosis
