摘要
目的 :探讨光活化的金丝桃素对人前列腺癌细胞 PC3M的抑癌作用。方法 :用生长抑制试验和流式细胞技术观察给予金丝桃素后 PC3M细胞的增殖、细胞周期和凋亡的变化。结果 :不同浓度的金丝桃素在体外可显著抑制 PC3M细胞增殖 (P<0 .0 5 ) ,药物浓度为 0 .5、 1 .0和 2 .0 m g· L- 1 时 ,抑制率分别为 2 1 %、 5 0 %和 91 % ;细胞周期出现 S期阻滞 ,由 S期进入 M2 期的比例为零 ,并诱导 PC3M细胞凋亡。结论
Objective To study the influence of hypericin on the proliferation and apoptosis of cultured human prostate cancer cells. Methods Growth inhibition test and flow cytometry were used to examine the changes of proliferation, cell cycle and apoptosis. Results Hypericin of different concentrations could significantly inhibit the proliferation and induce the apoptosis of human prostate cancer cells cultured in vitro . When the concentrations were 0 5,1 0,2 0 mg·L -1 ,the inhibitory rates were 21%,50%, and 91%,respectively. S phase was blocked and didn′t change into M 2 phase. Conclusion Hypericin can inhibit the proliferation and cell cycle of cultured human prostate cancer cell and induce its apoptosis.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第1期97-99,共3页
Journal of Jilin University：Medicine Edition
基金
日本 Jica项目资助课题 (科技部 5 9号 )
