摘要
对土壤中放线菌参与土壤反硝化可能性进行了初步研究。从土壤 10 - 5 稀释液中共获得 2 3个放线菌单菌落 ,初步鉴定属于 10个不同类型的菌株 ,其中 9个属于Streptomyces (链霉菌属 ) ,1个属于Actino madura (马杜拉属 )。 10个菌株在纯培养条件下都能将NO-3 还原成N2 O ,表明该土壤中具有反硝化能力的放线菌比例很高。测得其中 3个链霉菌菌株的N2 O产出速率为N 1 2～ 187 7μgg- 1min- 1(细胞干重基数 )。N2 O形成与放线菌生物量成正比。多数链霉菌菌株的N2 O产出受C2 H2 抑制。将 3个链霉菌菌株接种到灭菌土壤中厌气培养 ,均能测到N2 O形成 ,表明链霉菌可以利用土壤中原有碳源作为电子供体还原NO-3 。经历厌氧胁迫 2 1d以后 ,测试的
Twenty three single colonies of actinomycetes were iso lated from a soi l sample collected from Changshu Agroecological Experimental Station,Institute of Soil Science,Chinese Academy of Sciences Among 10 strains distinguished f ro m each other by morphological properties,9 were further identified as Strepto myces sps All strains showed capability of reducing nitrate into N 2O under anaerobic pure culture conditions Three strains were selected and denitrifica tion rates were measured Nitrous oxide production rate of these strains ranged from N 1 2 to 187 7 μg g -1 min -1 The strains were shown not only to grow well but also to be able to reduce nitrate to N 2O in sterile soil ,indicating that these strains were able to use soil organic carbon as electron donor for nitrate reduction Acetylene showed inhibitory effect on N 2O produ ction of most strains isolated Stressed under continuous strict anaerobic cond ition,strains of Streptomyces sps still showed considerable denitrification capacities
出处
《土壤学报》
CAS
CSCD
北大核心
2004年第1期108-112,共5页
Acta Pedologica Sinica
基金
国家自然科学基金项目 ( 30 1 70 0 2 9)
中国科学院南京土壤研究所土壤圈物质循环开放实验室基金课题
中国科学院知识创新工程项目(KZCX2 - 41 3- 3- 1 )资助
关键词
土壤
放线菌
反硝化性
土壤微生物
Actinomycetes
Denitrification
Soil microorganisms
