摘要
以红树植物白骨壤 (Avicenniamarina)基因组DNA为模板 ,根据超氧化物歧化酶基因保守序列设计特异引物进行PCR扩增 ,得到特异基因片段。回收该基因片段 ,与pMD18 T载体连接 ,并转化到感受态大肠杆菌ER2566细胞 ,获得铜锌超氧化物歧化酶基因片段的克隆。序列分析表明白骨壤铜锌超氧化物歧化酶基因片段含4个外显子和3个内含子 ,编码78个氨基酸,与水稻、玉米、红薯和白杨相应氨基酸序列的同源性分别为83.3% ,84.6% ,84.6%和87.2 %。
Special oligonucleotides to highly conserved regions of superoxide dismutases (SOD) gene were used to prime the synthesis and amplification of gene fragment from Avicennia marina genomic DNA by polymerase chain reaction (PCR). The fragment was linked with pMD18 T vector and transformed into the competence cell of Escherichia coli ER2566 strain. After screening of recombinants, detection and sequence analysis, the transformants containing SOD gene were obtained. Sequencing analysis showed that extracted from A. marina Cu·Zn SOD gene fragment,which contains four exons and three introns, had 767 nucleotides. The amino acid sequences which are homologus to those from Cryza sativa, Zea mays, Ipomoea batatas and Populus tremuloid are 83.3%, 84.6%, 84.6% and 87.2% respectively.
出处
《海洋科学》
CAS
CSCD
北大核心
2004年第1期43-47,共5页
Marine Sciences
