摘要
采用离体血管环技术比较了正常鸡、亚临床腹水鸡和腹水鸡的离体肺外动脉对乙酰胆碱(ACh)的内皮依赖性和对硝普钠(SNP)的非内皮依赖性舒张反应性。结果显示:在苯肾上腺素(PE)预收缩后,正常鸡、亚临床腹水鸡和腹水鸡的肺外动脉对ACh和SNP均表现出浓度依赖性舒张反应。低ACh和SNP浓度时,三者的舒张反应相差不明显,高浓度时,腹水鸡和亚临床腹水鸡的舒张反应性明显比正常鸡低。3 3×10-6ACh可使正常鸡肺外动脉舒张百分数达100%,亚临床腹水鸡和腹水鸡仅分别为83 12%和80 84%;3 3×10-7SNP使正常鸡肺外动脉舒张百分数达98 17%,亚临床腹水鸡和腹水鸡仅分别为70 46%和71 65%。亚临床腹水鸡和腹水鸡肺外动脉对PE的收缩反应比正常鸡强。结果表明肺动脉高压肉鸡肺外动脉对ACh的内皮依赖性和对SNP的非内皮依赖性舒张反应都下降,对PE的收缩反应性增加。结果初步证明肺外动脉舒张反应性下降及收缩反应性增加与肉鸡肺动脉高压的形成有关。
By using an in vitro vascular ring technique we compared the endotheliumdependent relaxation by acetylcholine (ACh) and ehdotheliumindependent relaxation by sodium nitroprussiate (SNP) of extrapulmonary arteries from normal,preascitic and ascitic broilers.After constricted with phenylephrine (PE),the vascular rings were all showed concentrationdependent relaxation with ACh and SNP.The vascular relaxation of normal,preascitic and ascitic broilers were similar at low ACh and SNP concentrations.But at higher concentrations,relaxations of preascitic and ascitic broilers extrapulmonary arteries to ACh and SNP were all lower than normal broilers.The percent relaxation of vascular rings by 33×10-6 ACh was 100% of normal broilers,and 8312% of preascitic,8084% of ascitic broilers respectively.The percent relaxation of vascular rings by 33×10-7 SNP was 9817% of normal,and 7046% of preascitic,7165% of ascitic broilers respectively.The contraction to phenylephrine of preascitic and ascitic broilers arteries were all higher than normal broilers.The results showed that the endotheliumdependent and ehdotheliumindependent relaxation of extrapulmonary arteries from broilers with pulmonary hypertension to ACh and SNP were all lower than normal broilers,but the contraction to phenylephrine were all increased.Our results suggested that the decreased relaxation to ACh and SNP and the increased contraction to phenylephrine might contribute to the development of pulmonary hypertension of broilers.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2003年第3期262-267,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
农业部九五攻关专题资助项目(95牧 02 03 11)
关键词
正常鸡
亚临床腹水鸡
腹水鸡
肺外动脉
舒张反应性
血管反应性
Broilers
Vascular reactivity
Pulmonary artery
Pulmonary hypertension syndrome
Ascites syndrome
