摘要
以质粒pBUSCK HPT作抗虫基因供体 ,优良籼型保持系D6 2B为受体 ,采用基因枪转化法 ,获得了转抗虫基因sck的植株。将转基因保持系与不育系D6 2A回交 ,获得转抗虫基因不育系。分子证据表明 ,外源基因能稳定转移到不育系中。蛋白活性测定显示 ,转基因在不育系中得到表达。
The transgenic D62B, an elite indica maintainer line, containing modified CpTI (sck) , was obtained by bombardment With the transgenic D62B as donor, D62A containing sck was developed PCR and Southern blotting analyses indicated that sck gene could be delivered to CMS line stably The field performance of transgenic plants was similar to the non transgenic control, but the resistance to insects was increased The use of genetic transformation in rice breeding was also discussed
出处
《作物学报》
CAS
CSCD
北大核心
2004年第1期60-65,共6页
Acta Agronomica Sinica
基金
国家"8 63"计划
国家转基因植物研究与产业化专项 (J2 0 0 0 B 0 11)
四川省"九五"生物技术攻关等项目资助
