摘要
利用SSR标记分析了 1 6份和 1 4份代表云南不同生态地区糯玉米、爆裂玉米地方种的遗传多样性。从 96对SSR引物中分别筛选出分布于玉米基因组 1 0条染色体上的 6 1对引物和 4 3对引物 ,每对引物可以分别稳定地检测到 1～1 2个和 1～ 1 3个多态性片段 ,糯玉米共 2 2 6个 ,平均为 3.70个 ,片段大小介于 70～ 70 0bp之间 ;爆裂玉米共 2 32个 ,平均检测的多态性片段为 5 .4 0个 ,片段大小介于 6 5～ 4 90bp之间。糯玉米中检测出 1个等位基因 (allele)的引物有 9对 ,占所筛选引物的 1 4 7% ;检测出 1 1、1 2个等位基因的引物有 2对 ,占所筛选引物的 3 3% ;其余 5 0对引物检测出的等位基因一般在 2～ 7个 ,占所筛选引物的 82 0 %。爆裂玉米中能检测出 1个等位基因 (allele)的引物有 3对 ,占所筛选引物的7 0 % ;能检测出 1 2个和 1 3个等位基因的引物有 3对 ,占所筛选引物的 7 0 % ;其余 37对引物能检测出的等位基因一般为 2～ 9个 ,占所筛选引物的 86 .0 %。上述结果表明云南糯玉米、爆裂玉米地方种质具有较高的遗传多样性。通过聚类分析将云南糯玉米分为 3个类群和 5个亚群 ,云南爆裂玉米分为 3个类群和 4个亚群。
waxy corn landraces and 14 popcorn landraces in Yunnan were analyzed by SSR marker for genetic diversity. 61 primers and 43 primers were screened to be specific with chromosome 10 of waxy corn and popcorn respectively from 96 primers tested. Polymorphic fragments were amplified stably by each specific primer, 1-12 for waxy corn and 1-13 for popcorn. In total 226 specific DNA bands of 70-700 bp in size were detected in waxy corn with an average of 3.70 fragments for each primer. 9 primers accounting for 14.7% in the primers used could detect 1 allele, 2 primers accounting for 3.3% could detect 11 and 12 alleles respectively. Other 50 primers accounting for 82.0% could normally detect 2-7 alleles. For popcorn landraces, 232 alleles of 65-490 bp in size were detected in total, 5.40 alleles by each primer on average. 3 primers accounting for 7.0% could detect 1 allele, other 3 primers accounting for 7.0% could detect 12 and 13 alleles respectively. The rest of 37 primers accounting for 86.0% could usually detect 2-9 alleles. The results suggested that higher genetic polymorphism was maintained in local waxy corn and popcorn landraces of Yunnan. The result by cluster analysis showed that waxy corn could be divided into 3 groups and 5 sub groups, and popcorn into 3 groups and 4 sub groups. That was generally in accordance with the tendency of topography and altitude in Yunnan.
出处
《作物学报》
CAS
CSCD
北大核心
2004年第1期36-42,共7页
Acta Agronomica Sinica
基金
教育部西部高级访问学者计划项目资助
