摘要
目的 :用RP HPLC法研究多舌飞蓬 [Erigeronmultiradiatus(Wall .)Benth .]中主要成分灯盏乙素在不同产区 ,不同采收季节 ,个体器官之间的变化规律。方法 :测定的样品用甲醇 水 (1∶1)提取 ;色谱柱ShimadzuODS 18(150× 4.6mm ,5μm) ,流动相∶甲醇 水 醋酸 (3 9∶61∶0 .6) ,流速 0 .8ml/min ,检测波长 3 3 5nm ,柱温 3 5℃。 结果 :不同地区 ,不同季节的多舌飞蓬中的灯盏乙素的含量不同 ,地上部分远高于地下部分。结论
AIM: To study the regular change of scutellarin in Erigeron mul tiradiatus (Wall.) Benth, from different areas, different seasons and differ ent parts of plant by means of RP-HPLC. METHODS: The herbs of Erigeron multira diatus were extracted with CH 3OH-H 2O(1∶1) wixture, The sa mples were separated on an ODS column with the mobile phase CH 3OH-H 2O-HAC(39∶61∶0.6). The flow rate was 0.8ml/min, the detection wavelen gth was set at 335nm and the temperature was at 35℃. RESULTS: The determination of scultellarin was different from diffe rent areas and different seasons, Scutellarin content of terraneous including stem、leaf and flower was higher than that of subterraneous root. CONCLUSION: Variation in Erigeron multiradiatus was influenced by the combined effects of genotype and ecological conditions.
出处
《中成药》
CAS
CSCD
北大核心
2004年第1期60-62,共3页
Chinese Traditional Patent Medicine
