摘要
目的 :建立秦艽药材HPLC指纹图谱。方法 :采用梯度洗脱法 ,对秦艽药材进行HPLC测定 ,流动相为甲醇 -0 .4%磷酸线性梯度洗脱 ,检测波长为 2 3 0nm ;记录时间 :70min。采用浙江大学出版的指纹图谱相似度比较软件进行比较。结果 :运用梯度洗脱能很好分离秦艽的各类成分 ,通过软件的比较 ,秦艽药材的指纹图谱相似度均大于 0 .90。结论
AIM: To establish the HPLC fingerprint of Genti ana straminea Maxim. METHODS: It was adopted gradient with Methanol and 0.4% phospho ric acid, the detection wavelength was at 230nm and recording time. And it w as compared with a software published by Zhejiang University. RESULTS: The constituents of Gentiana straminea Maxim. were well se parated by HPLC, and the similarity was more than 0.90 . CONCLUSION: The method can be used as the fingerprint of G entiana straminea Maxim.
出处
《中成药》
CAS
CSCD
北大核心
2004年第1期4-7,共4页
Chinese Traditional Patent Medicine
关键词
秦艽
HPLC
指纹图谱
Gentiana straminea Maxim
HPLC
fingerprint
