摘要
目的 分析转染醛糖还原酶相似基因 1(ARL 1)后 ,实验组人肝癌细胞与对照组细胞在基因表达谱方面的差异。 方法 以实验组及对照组细胞的总RNA反转录合成的cDNA为探针 ,与cDNA芯片 (cDNAchips)进行差异杂交 ,并应用半定量RT PCR对差异表达的重要的相关基因进行初步筛选。 结果 cDNA芯片分析发现 6种基因的表达水平明显上调 ,9种基因的表达水平明显降低 ,涉及细胞的蛋白质代谢、信号传导、转移和耐药性等方面。 结论 cDNA芯片分析发现 :转染醛糖还原酶相似基因 1后肝癌细胞的基因表达谱发生改变 ,为系统研究肝癌的耐药性及其机制提供了有益的线索。
Objective To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene. Methods The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Results Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway,metastasis, and drug resistance. Conclusions cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2003年第3期180-182,共3页
Chinese Journal of Surgery
