摘要
用Tb^(3+)分别对用和未用顺二氨二水合铂(Ⅱ)(AAP)处理的红细胞膜和磷酰胆碱(PC)脂质体进行荧光滴定,测Tb^(3+)荧光。用AAP滴定红细胞膜,测蛋白质自身荧光变化。用间接Scatchard法求结合参数。表明蛋白质及磷脂均与AAP结合,蛋白质结合能力较强。
The interactions between cis-diaquidiammineplatinum(II), AAP and membrane proteins and phospholipids of erythrocytes were studied by titration of the crythrocyte membrane, intact and AAP treated, and phosphotidylcholine liposomc with Tb3+ by monitoring Tb3+ fluorescence. The membrane was also titrated with AAP by measuring the intrinsic fluorescence of protein. The binding parameters are obtained by Scatchard method. The results show that AAP binds to both proteins and phospholipids, but stronger with proteins.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
1992年第3期225-229,共5页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金
国家教委博士点基金
关键词
膜磷脂
膜蛋白
AAP
荧光光谱
cis-diaquodiammineplatinum(II) membrane phospholipids embrane protein
