摘要
目的 研究甲状旁腺素 (PTH)对心肌细胞内游离钙以及细胞肥大和凋亡的影响。方法 利用培养的新生大鼠心肌细胞 ,以Fluo 3/AM负载 ,通过激光共聚焦显微镜(LSCM)测定细胞内游离钙浓度 ([Ca2 + ] i) ;以细胞面积和细胞蛋白含量作为心肌细胞肥大指标 ;采用电镜和流式细胞术观察细胞凋亡的变化。结果 PTH1～ 34 0 0 1和 0 1 μmol·L- 1 刺激 7d后 ,心肌细胞内钙荧光强度以及心肌细胞面积和蛋白含量、细胞凋亡率较对照组显著增加。而 0 1 μmol·L- 1 PTH1～ 34 刺激的同时分别加入 1、1 0 μmol·L- 1 硝苯地平 ,上述指标改善 ,但未能达正常。结论 PTH1～ 34 可显著增加心肌细胞 [Ca2 + ] i,诱导细胞肥大和凋亡 ,并呈浓度依赖性 。
AIM To study the effects of parathyroid hormone(PTH) on intracellular calcium concentration([Ca 2+ ] i), hypertrophy and apoptosis in rat cardiomyocytes. METHODS Neonatal rat cardiomyocyte hypertrophic response was assayed by measuring cell surface area and protein content. [Ca 2+ ] i was measured by confocal microscope using Fluo 3/AM as fluorescent indicator.Apoptosis was determined by flow cytometer and electron microscope. RESULTS After 7 d exposure to PTH 1～34 of 0 01 and 0 1 μmol·L -1 ,[Ca 2+ ] i,surface area and protein content,and apoptosis rate(APO) of cardiomyocytes were significantly increased,especially in high concentration groups. While application of both nifedipine and PTH 1～34 significantly decreased lose indicators, they were still markedly higher than normal. CONCLUSIONS PTH 1～34 dose dependently induces hypertrophy and apoptosis of cardiomyocyte,and this is attributed able to augmented entry of calcium into cell via voltage dependent Ca 2+ channel.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第7期826-829,共4页
Chinese Pharmacological Bulletin
基金
20 0 1年江苏省普通高等学校"青蓝工程"启动基金No 2 0 0 1 0 9
关键词
甲状旁腺素
心肌细胞
细胞凋亡
游离钙
parathyroid hormone(PTH)
cardiomyocytes
apoptosis
calcium
