摘要
目的 探讨肝移植术后急性排斥反应的早期诊断方法。方法 应用RT PCR技术检测大鼠肝移植术后胆汁中的IL 6和IFN γ基因表达 ,以组织病理学作为急性排斥反应的诊断标准 ,将肝移植大鼠分为急性排斥组 (Rt组 )和非急性排斥组 (Rf组 ) ,观察胆汁中IL 6和IFN γ基因表达与急性排斥反应的关系。结果 胆汁中IL 6基因表达在Rt组和Rf组无显著性差异 (P >0 0 5 ) ,而IFN γ基因表达在Rt组和Rf组有显著性差异 (P <0 0 1)。结论 检测胆汁中IFN γ基因表达可能是早期诊断肝移植术后急性排斥反应的一个有效指标 ,而IL 6基因表达则无助于急性排斥反应的诊断。
Objective To determine the method for early diagnosis of acute rejection (AR) following liver transplantation. Methods RT-PCR was used to detect the gene expression of interleukin-6 (IL-6) and interferon-γ (IFN-γ) in bile for diagnosis of AR after liver transplantation in rats. The rats receiving liver transplantation were divided into the Rt group (with AR) and Rf group (without AR). Then the relationship between the gene expression of IL-6 and IFN-γ in bile and AR was observed. Results There was no significant difference in gene expression of IL-6 between the 2 groups (P>0.05). However, the gene expression of IFN-γ was significantly different between the 2 groups (P<0.01). Conclusions The gene expression of IFN-γ in bile might be a useful indicator for early diagnosis of AR after liver transplantation, but the gene expression of IL-6 might be helpless to diagnosis of AR.
出处
《中华肝胆外科杂志》
CAS
CSCD
2003年第9期550-553,共4页
Chinese Journal of Hepatobiliary Surgery
基金
卫生部临床学科重点项目资助 (基金编号 3 9470 714 )
