摘要
目的 研究宫颈癌组织中是否存在DNA聚合酶 β(POLB)基因的突变。 方法 采用逆转录 聚合酶链反应 (RT PCR)技术、聚合酶链 单链构象多态性分析 (PCR SSCP) DNA序列分析法对34例宫颈癌组织进行POLB基因突变的调查。结果 宫颈癌组织中存在POLB基因突变 ,且基因突变率与癌组织的病理分级有关 ,G1、G2 、G3 宫颈癌组织中POLB基因突变率分别为 9例中 1例 ,2 9%(4/ 14 )、73% (8/ 11) ,除G1与G2 比较 ,差异无显著性外 (P >0 0 5 ) ,余两比较 ,差异均有极显著性 (P<0 0 0 5 )。测序结果表明 ,POLB基因的第 6 6 0位核苷酸由A变为G ,其氨基酸的第 182位由精氨酸(Arg)变为甘氨酸 (Gly)。结论 宫颈癌组织中存在POLB基因突变现象 ,可能与宫颈癌的发生、发展相关。
Objective To investigate whether DNA polymerase β(POLB) gene mutations occur in human cervical cancer (CC). Methods To collect fresh specimens from 34 cervical cancer and examine the mutation of POLB gene using reverse transcription polymerase chain reaction(RT-PCR),single strand conformation polymorphism(SSCP) analysis and sequence analysis.According to their histological grading, the 34 cases of CC were divided into three groups: 9 cases of grade Ⅰ(G 1),14 cases of grade Ⅱ(G 2),11 cases of grade Ⅲ(G 3). Results POLB mutations were detected in the tissues of CC.The mutation of POLB is related with the histological differentiated of the CC.The mutated rates in low differentiated cancer were significantly higher than those in moderate and high ones, and the difference was significant (P<0.05).The sequencing of the PCR product showed an A to G chang at nucleotide 660,which resulted in a substitution of the amino acid from arginine to glycin at codon 182. Conclusions There were POLB mutations in the tissues of CC ,and this may be related with the development of the CC.The mutations impaired the catalytic activity of POLB.As a result ,this may lead to observed accumulation of mutations in tumor cells.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2003年第10期618-620,共3页
Chinese Journal of Obstetrics and Gynecology
