摘要
目的 筛选研制人工食管的最适生物材料 ,探讨其特殊的成型工艺 ,并通过动物模型了解其用于食管缺损重建的可行性 ,为临床研究提供实验依据。 方法 根据理想化食管替代物的要求 ,设计出聚氨酯 胶原蛋白壳聚糖复合人工食管 ,由医用聚氨酯制成直管状内管 ,外壁采用胶原蛋白 壳聚糖多孔状海棉覆盖。将 1 3只成年健康杂种犬分为 2组 ,以聚氨酯 胶原蛋白壳聚糖复合人工食管为实验组 (8只 ) ;硅橡胶管作为对照组 (5只 ) ,构建犬颈段食管置换与重建的动物模型。 结果 实验组犬术后人工食管假体能迅速被宿主周围组织包绕达到生物学固定 ;内腔可见不规则黏膜上皮生长 ,1个月后覆盖内腔全长 ;吻合口肉芽组织生长不明显 ,动物存活达 1 2个月的 1只 ,现仍存活良好。对照组犬术后人工食管内腔未见黏膜上皮生长 ,动物均在术后 2个月内死亡。 结论 聚氨酯 胶原蛋白壳聚糖生物材料复合人工食管可应用于食管置换重建手术 。
Objecctive To design and develop a novel esophageal prosthesis by selecting appropriate biomaterials, developing special manufacturing techniques, and investigating the feasibility of replacement of cervical esophagus in mongrel dogs. Methods In accordance with the requirements of ideal esophageal substitutes,we designed a new type of esophageal prostheses.The inner stent wae made with polyurethane of medical grade, and the outter surface of the prosthesis was coated with collagen chitosan sponge. The silicone tube was used as a control. Thirteen adult mongrel dogs that were divided into two groups were used to establish the experimental models. Results In the experimental group( n =8),the esophageal prostheses were completely incorporated with the native esophagus and adherent to the surrounding host connective tissues. Epithelial linings of varying degrees were formed on the luminal surface, and complete epithelization was seen in 1 month postoperatively. The granulation at the sites of the anastomosis in this group was less significant than that of the control group. One dog has been surviving for 12 months up to now without any complications. In the control group( n =5), esophageal epithelial was not observed on the luminal surface, constriction of the regenerated esophagus progressed and all the dogs died within 2 months after operation. Conclusion These observations suggest that this esophageal prosthesis made of composite biomaterials has high biocompatibility and potential for long segment esophageal reconstruction,which is promising for the clinical repair of esophageal defects.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2003年第7期541-544,共4页
Chinese Journal of Surgery
基金
上海市科学技术发展基金资助项目( 0 0 4 1190 0 3)
关键词
生物材料
人工食管
设计
制备
动物实验
生物相容性
Esophagus
Artificial organs
Biocompatible materials
Esophageal reconstruction
Model,animal
