摘要
目的探讨两种促炎性细胞因子γ-干扰素(Interferon-γ,IFN-γ)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在视神经炎(optic neuritis,ON)免疫学发病机制的作用,研究二者之间的产生特点、变化规律及相互关系。方法采用三色荧光抗体染色技术经流式细胞仪,检测了20例ON患者外周血(peripheral blood,PB)中CD3+T细胞内TNF-α及IFN-γ,并对活动期和缓解期ON患者及甲基强的松龙(methylprednisolone,MP)冲击疗法治疗前、后的ON患者PB中,CD3-T细胞内TNF-α和IFN-γ进行了比较。结果(1)活动期ON,PB中CD3+T细胞内TNF-α及IFN-γ较其他神经疾病组(other neurological disease,OND)明显增加(P<0.01),较正常对照组(NO)亦明显增加(P<0.01,P<0.05);(2)TNF-α与IFN-γ呈正向直线相关的关系(r=0.608,P<0.01);(3)TNF-α与病情严重程度有关(P<0.05);(4)缓解期ON,PB中TNF-α及IFN-γ与活动期比较明显减少(P<0.01,P<0.05).与NC组比较TNF-α明显减少(P<0.05),IFN-γ则无明显变化(P>O.05);(5)MP冲击疗法治疗后,TNF-α及IFN-γ均较治疗前明显降低(P<0.01)。结论TNF-α和IFN-γ作为二种重要的促炎性细胞因子参与了ON的免疫病理过程;二者作用协同,在促进、促发炎症、上调疾病方面发挥重要作用。TNF-α和IFN-γ虽不是ON的?
Objective To investigate the characteristics of two pro-inflammatory cytokines (CKs), tumor necrosis factor-a (TNF-α) and interferon-γ (IFN-γ)in the pathogenesis of optic neuritis (ON), and the alteration and interaction between them. Methods By the flow cytometric analysis, a three-colour- immunofluorescent staining of cell surface antigen and intracellular cytokines (TNF-α and IFN-γ) in CD3+ T-lymphocytes of 20 patients with ON, other neurological disease (OND) were performed, as well as normal control (NC) . We also compared the difference of number of TNF-α staining T-cells and IFN-γ staining T-cells between the acute and remission stage, before and after methylprednisolone (MP) therapy. Results (1) ON patients in the acute stage had more TNF-αstaining T cells and IFN-γ staining T cells in PB than OND(P<0.01) or NC group (P<0.01, P<0.05). (2) In the acute stage, the number of TNF-α staining T cells was closely related to the number of IFN-γ staining T cells(r=0.608, P<0.01). (3) In the acute stage, the number of TNF-α staining T cells of the severe ON patients was significantly higher than that of the mild patients (P<0.05). (4) The number of TNF-α staining T cells of patients in remission stage was significantly lower than that of patients in acute stage(P<0.01), and was lower than normal control group (P<0.05)as well. (5) After MP therapy, the number of TNF-α staining T cells and the number of IFN-γ staining T cells were significantly lower than that before MP therapy(P<0.01). Conclusion TNF-α and IFN-γ, the two important pro-inflammatory CKs, may be involved in the immuno-pathologic process of ON. The number of TNF-α staining T cells and the number IFN-γ staining T cells, are useful for monitoring the activity of ON, although they are not specific markers of ON.
出处
《中华神经医学杂志》
CAS
CSCD
2004年第1期25-27,共3页
Chinese Journal of Neuromedicine
