摘要
目的研究乙酰胆碱酯酶(AChE)重活化剂与正常和中毒AChE分子间作用力。方法原子力显微技术(AFM)。结果PAM-Cl、OBD和HI-6与正常AChE粘附力分别为28±2 pN、40±3 pN和52±6 pN,与沙林中毒AChE间粘附力可分别增加至830±120 pN、1099±143 pN和1544±98 pN,为正常AChE的30、27和30倍;与VX中毒AChE间粘附力可分别增加至928±122 pN、1375±143 pN和1288±11.2 pN,为中毒前的25、30和26倍;对梭曼中毒AChE没有重活化作用的PAM-Cl和OBD与梭曼中毒AChE粘附力没有明显增加;对梭曼中毒酶有重活化作用的HI-6与梭曼中毒AChE间粘附力为854±86 pN,为中毒前16倍。结论重活化剂-中毒AChE分子间粘附力与药物重活化作用一致,是重活化作用的力学基础。
Objective To study the intermolecular forces between normal and intoxicated acetylcholinesterases (AChE) and reactivators (PAM-C1, OBD and HI-6). Methods The intermolecular forces were observed with atomic force microscopy. Results The intermolecular adhesions between NA-iAChE and the three reactivators were much stronger than that before the AChE were intoxicated. The adhesions between the three reactivators and sarin-intoxicated AChE were respectively 830?20 pN, 1099?43 pN and 1544?8 pN, and 30-, 27- and 30-fold of that before intoxication. The adhesions between the three reactivators and VX-intoxicated AChE were respectively 928?22 pN, 1375?43 pN and 1288?1.2 pN,and 25-, 30- and 26-fold of that before intoxication. There was no significant increase in the adhesion between soman-intoxicated AChE and PAM-C1 and OBD, which have no significant reactivating effects while there was indeed a significant increase in the adhesions between soman-intoxicated AChE and HI-6, which has reactivating effects on soman-intoxicated AChE. Conclusion The intermolecular adhesive forces between reactivators and NA-iAChE were quite in consistence with the reactivating effects of the reactivators, suggesting that the strong adhesions between NA-iAChE and the reactivators were the force basis for the reactivating effects of the reactivators on NA-iAChE.
出处
《中华神经医学杂志》
CAS
CSCD
2004年第1期11-13,21,共4页
Chinese Journal of Neuromedicine
基金
国家自然科学基金(39780041
30271501)
军队"十五"规划科研基金重点课题(01Z024)
关键词
乙酰胆碱酯酶
活化剂
ACHE
重活化剂
atomic force microscopy
acetylcholinesterases
intermolecular forces
reactivators
