摘要
通过对赤毒素、竹红菌甲素及苯酚量子产率的测定与比较发现,这三种荧光化合物都具有一个相对于激发波长的量子产率的稳定区域。尽管它们具有多个激发峰,但不同激发峰所激发的荧光量子产率差别较小。竹红菌甲素在室温放置一个月,690nm荧光光谱有明显的改变。以上结果提示在测定未知荧光化合物的量子产率时,被测溶液的散射较强,同时荧光物的激发与发射波长彼此相接近。量子产率较弱时,可以在最大激发峰的蓝移方向上选择激发波长来避免散射光的干扰,提高量子产率测定的准确度。竹红菌甲素在690nm的荧光肩峰,可能是分子空间结构上容易发生化学变化的部位所发射的。
Determination of the quantum yields of Gibberellin, Hypocrellin A and phenol showed that there were respectively ranges of excitation wavelengths for the relative constant of quantum yields of them. The quantum yields of Gibberellin excited by its own multiple excitaion wavelengths (285 and 410 nm) equaled each other. The quantum yields were similar by excitation of 585 nm and 625 nm with determination of the emission at 640 nm for Hypocrellin A. The emission at 690nm with Hypocrellin A would disappear after it had stood at room temperature for about one month, it suggests that it should be convenient to determine the quantum yield, at a shorter wavelength as for the excitation maximum, of a fluorophore whose concentration was low, weak quantum yield and wavelengths of the excitation and emission locating nearly, with a strong Reilagh scattering; and that the 690 nm emission of Hypocrellin A should be emitted by the groups which were subjected to the effects of physical or chemical disturbences.
出处
《微生物学通报》
CAS
CSCD
北大核心
1992年第6期340-344,共5页
Microbiology China
关键词
赤霉素
竹红菌甲素
荧光量子产率
Gibberellin
Hypocrellin A
Fluorescence
Quantum yield
