摘要
分别从解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和金黄色葡萄球菌(Staphylococcus aurcus)中提取染色体DNA,经HindⅢ酶解后PCR扩增获得Barnase(核酸酶BN)基因和Staphylococcal Nuclease(核酸酶SN)基因,并克隆到质粒pGEMTZ-f(+)上。序列分析表明,核酸酶BN的核苷酸序列与已发表的序列有99.3%的同源性,而与据此推测的氨基酸序列完全一致。核酸酶SN基因的核苷酸序列与已发表的序列有99.1%的同源性,与据此推测的氨基酸序列有99.3%的同源性。该工作为利用核酸酶的特异表达或激活获得雄性不育植物及探索新的抗病毒策略打下了基础。
Total DNA of B. amyloliquefaciens and S. aureus were isolated and cut partially with Hind Ⅲ Separately, The genes for barnase and for staphylococcal nucl ease were obtained by PCR and cloned into pGEM7Z-f(+), The sequencing show that the n ucleotide (nt) sequence of the barnase is 99.3% homologous with the previously determimed sequence and the deduced animo acid (an)sequence found to correspond precisely to the previously determined sequence; the nucleotide (nt) sequence of the staphylococcal nuclease is 99.1% homologous with the previously determined sequence and the deduced amino acid (an) sequence 99.3% homologous with the previously determinded sequence.
These have built basis of making use of special expression or activation of nuclease to produce the male sterility plant by plant gene engineering and to explore new antiviral strategy.
出处
《微生物学通报》
CAS
CSCD
北大核心
1992年第4期200-203,共4页
Microbiology China
