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不同剂量rhGM-CSF cDNA直接注射小鼠骨骼肌的表达 被引量:1

Various Amounts of rhGM - CSF cDNA Transfer Directly into Mice's Muscle in vivo
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摘要 将表达人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)的重组真核表达质粒(pCG1),以30μg和60μg的剂量分别直接注射小鼠骨骼肌,观察不同剂量的质粒(pCG1)在小鼠体内分泌表达rhGM-CSF的情况。ELISA检测表明,注射60μg质粒DNA的小鼠血液中测得的rhGM-CSF含量与对照组相比有显著差别,而注射30μg质粒DNA的小鼠血液中rhGM-CSF含量与对照组相比无显著差别。以60μg质粒DNA直接注射骨骼肌后第15~20天左右表达量最高,平均约91ng/ml。生物学活性检测结果显示,注射60μg质粒DNA的小鼠血液能维持GM-CSF依赖的TF-1细胞的生长。这提示用质粒DNA直接注射小鼠骨骼肌时,质粒DNA必须达到足够的量。 We have transferred the different amounts of the plasmid DNA pCGl, which was constructed by cloning rhGM-CSF gene into the eukaryotic expression plasmid pCDS, directly into the BALB/c mice's quadriceps pretreated with bupivacaine. The result of ELISA confirmed that rhGM - CSF was expressed and secreted in mice of group B injected 60μg of plasmid pCGl, but not in mice of group C injected 30μg of plasmid pCGl. The time for the highest level of rhGM-CSF expression was during the fifteenth to twentieth days after plasmid DNA pCGl was injected. Meanwhile, the results of biological activity detection showed that mice's serum of group B (injected 60μg) had the function of retaining the growth of TF - 1 cells. This demonstrates that the rhGM-CSF in mice's serum of group B has certain biological activity. This Model of animal will blaze a new trail in the treatment and prevention of the leukyopenia.
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 1996年第3期226-229,共4页 Chinese Journal of Cancer Biotherapy
关键词 RHGM-CSF CDNA 直接注射 小鼠 骨骼肌 表达 基因治疗 基困转染 gene therapy GM - CSF gene transfer skeletal muscle
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  • 1麦跃,国外医学遗传学分册,1993年,2卷,66页
  • 2谭骏,科学通报,1993年,38卷,1918页

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