摘要
将巨大芽孢杆菌（Bacillus megaterium）α-淀粉酶基因克隆到短小芽孢杆菌（Bacilluspumilus）中获得表达。将该工程菌发酵液的上清液,经硫酸铵分级盐析和 DEAE-纤维素柱层析,得到纯化的α-淀粉酶。此酶的最适 pH 为6.0;在 pH 5—8之间稳定;最适反应温度为55℃;金属离子 Zn2+、Ab2+、Cu2+、Ag+对酶有明显的抑制作用;Ca2+、Na+、K+对酶略有激活作用;3×10-3mol/L 对氯汞苯甲酸（PCMB）对酶有95%的抑制作用;其免疫性质与枯草芽孢杆菌（Bacillus subtilis）所产生的α-淀粉酶相同。
The gene of α-amylase of Bacillus megaterium was cloned and expressed inBacillus pumilus.The purified enzyme was obtained by salting out of(NH_4)_2SO_(?)and DEAE-cellulose chromotography.The optimum pH of enzyme is 6.0.It is stablebetween pH 5—8.The optimum temperature is 55℃.The activity of enzyme wasinhibited by Zn^(2+),Ab^(2+),Cu^(2+),and a little activated by Ca^(2+),Na^+、K^+.When PCMBwas added at the concentration of 3×10^(-3)mol/L,95% of enzyme activity wasinhibited.The properties of immunology of enzyme are the same as that of α-amylase produced Bacillus subtilis.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第6期400-404,共5页
Acta Microbiologica Sinica
关键词
短小芽孢杆菌
淀粉酶
Bacillus pumilus 289
α-amylase
Immunology
