摘要
本文报道采用工程转座子 MudJ(lacZ,Kan^r)(以下简称 MudJ)诱变分离 add∷MudJ插入突变体和遗传定位的结果。从鉴别20000个 Kan′转导子中获得了6株add∷MudJ 突变体。酶活性测定表明,这些突变体无一有可检测的腺苷脱氨酶活性。转导分析证明,add 与pm(?)基因和与 purR 基因有10%连锁的。xx1900∷Tn10d-tet 插入物分别有70%和37%的共转导。三点杂交的结果确定 add 位于 pm(?) 和 zxx1900∷Tn10d-tet 之间,基因顺序为pmi(31.5′)-add-zxx1900∷Tn10d-tetpur R(30′)
Report here is the isolation of adenosine deaminase deficient mants and genetic mapping.Engineering transposon MudJ(lacZ,Kan^r)was used for mutagenesis and six add::MudJ wereobtained among 20 000 Kan^r transductants.Adenosine deaminase activity of these mutantswere assayed and all are negative Cotransduction analysis of add::MudJ indicatedthat add is 70% linked to pmi(31′)and 37% linked to zxx1900::Tn10d-tet insertion which is 10%linked to purR(30′).Three points cross showed that add is located between pmi and Ta10d-terinsertion.Therefore the gene order is purR-zxx1900::Tn10d-(?)et-add-pmi.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第5期328-333,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金
关键词
嘌呤
生物合成
鼠伤寒菌
Salmonella typhimurium
Adenosine deaminase deficient mutant
Genetic mapping
Engineering transposon
