摘要
比较了广谱型花生根瘤菌97-1菌株在花生和大豆根瘤中的形态分化和类菌体的繁殖能力。结果表明,97-1菌株在花生根瘤中分化为典型的球状类菌体,未观察到它们的繁殖,根瘤小杆菌的繁殖率也很低,并表现出随瘤龄增加而加大的趋势,渗透压保护条件对幼龄根瘤小杆菌的繁殖有一定影响。但在大豆根瘤中却分化为典型的杆状类菌体,其繁殖能力随瘤龄加大而增加,并且不需要渗透压保护条件。本研究结果直接证明,宿主植物影响根瘤菌在根瘤中的形态分化和繁殖特性。
The morphological differentiation and reproductivity of bacteroids isolated from nodulesof two representative legume plants,peanut and soybean,infected by a broad host range rhizo-bium strain 97-1,were studied.Typical round shaped bacteroids were formed and their co-lony-forming ability(CFA)was not observed with the symbiosis between 97-1 and peanut.TheCFA of small rods from nodules was very low but increased with the age of nodules.Osmo-protection showed a favorable effect on the CFA of small rods from young nodules.Typicalrod shaped bacteroids were also formed in soybean nodules infected by 97-1.Their CFA wasalso increased with the age of nodules but showed a detrimental effect with the osmoprotection.The determinative effect of plants on the morphological differentiation and reproductivity ofrhizobium bacteroids in nodules was directly proved by our results.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第4期278-284,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金
关键词
花生根瘤菌
形态分化
繁殖能力
Bradyrhizobium sp.(Arachis)
Morphological differentiation
Repoductivity