摘要
利用纸片显色方法,从土壤中快速筛选出98株产胞外青霉素酰化酶的菌种,经复筛其中10株酶活力较高,经鉴定均属于巨大芽孢杆菌。经单株分离得46号菌,用这株菌进行了产酶条件的研究,在最适产酶条件下,酶活力比开始提高了3.6倍。在此基础上又进行了物理化学因素处理,得突变株 UL-81,酶活力达720u/100ml 发酵液。对原株和突变株进行比较,发现UL-81菌落、细胞形态、诱导剂苯乙酸用量及添加时间等明显不同于原株。在500L 罐发酵酶活达820u/100ml 发酵液,为开始酶活的16倍。
Ninety-eight strains having extracellu-lar penicillin acylase activity were derivedfrom soil samples by colour-developing me-thod 10 strains of them possess higher acti-vity of penicillin acylase.All of those arefound to be Bacillus megaterium.The opti-mum condition of enzyme production wasinvestigated with the strain No.46 which isfrom No.247 by single colony isolation.Theproductivity of penicillin acylase in the op-timum condition have been enhanced 2.5times more than that in the screening condi-tion.The mutant strain,Bacillus megateriumUL-81,which penicillin acylase activity rea-ched the level of 723u/100ml of broth wasobtained from No.46 by treatment with phy-sical and chemical factors.The penicillinacylase activity of UL-81 can reach 820u/100ml in 500L fermentor.The mutant strain differed from parentstrain in the morphology of colony,the sizeof cells,the effect of concentration and theaddition time of phenylacetic acid on theproduction of penicillin acylase.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第2期99-104,共6页
Acta Microbiologica Sinica
基金
国家七五科技攻关项目
