摘要
用电泳检测 VTMoV88感染绒毛烟后病毒特异性核酸组份,发现核酸的小分子 RNA(卫星 RNA)组成发生变化。提纯的病毒通过电镜观察及血清学鉴定并与 VTMoV 加以比较,证明两者在颗粒形态、大小及血清学关系上一致。以克隆的 VTMoV-RNA3 cDNA 片断为探针检测 VTMoV 与 VTMoV88卫星 RNA 分子之间的核苷酸序列同源性,结果显示出两种毒源的卫星 RNA 具有高度同源性。据此,推测 VTMoV88可能是卫星 RNA 发生变异的突变体,并对 VTMoV88卫星 RNA 突变体形成机制进行了初步探讨。
It was found that the composition ofthe low-molecular-weight RNAs of VTMoVfrom different two origin VTMoV and VT-MoV88 varied by PAGE analysis.A compa-rison of purified VTMoV88 with VTMoV byelectron microscope and by serological reac-tions shows that VTMoV and VTMoV88 areidentical in the size and shape of both viralparticals and in serology.Using cloned cDNA3 as probe for detection of the satellite RNAs.of VTMoV88 and VTMoV were closely ho-mologous.The results above indicated VTMoVcould be a mutant with only satellite RNAsvariation.It was suggested that processing inreplication of RNAs might be the cause ofVTMoV RNAs variation.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第1期6-10,共5页
Acta Microbiologica Sinica
