摘要
目的 研究幽门螺杆菌 (Hp)源抗菌肽 (2 2 0 )对胃黏膜上皮细胞及胃癌细胞生长的影响。方法 采用琼脂扩散法检测Hp抗菌肽 (2 2 0 )及杀菌肽 (cecropin)B的抗菌活性及致死浓度 (LC) ,MTT法测定Hp抗菌肽 (2 2 0 )、杀菌肽B对胃黏膜上皮细胞株GES 1、胃癌细胞株SGC 790 1的细胞毒作用及半数抑制浓度 (IC50 )。结果 Hp抗菌肽 (2 2 0 )及杀菌肽B对E .coliK12D31的LC分别为 6 5和 0 .4 4μmol/L ,Hp抗菌肽 (2 2 0 )的抗菌活性较杀菌肽B低。Hp抗菌肽 (2 2 0 )及杀菌肽B对胃癌细胞具有浓度依赖性细胞毒作用。而Hp抗菌肽 (2 2 0 )在相对较低浓度时对胃黏膜上皮细胞有促进增殖的作用。杀菌肽B在 5～ 2 0 μmol/L浓度范围内对胃黏膜上皮细胞株GES 1无细胞毒作用。Hp抗菌肽 (2 2 0 )对胃黏膜上皮细胞株GES 1、胃癌细胞株SGC 790 1的IC50 为 6 30、5 0 0 μmol/L ,杀菌肽B对胃黏膜上皮细胞株GES 1、胃癌细胞株SGC 790 1的IC50 为 75、2 5 μmol/L。 结论 Hp抗菌肽 (2 2 0 )的抗菌活性较杀菌肽B低 ,对胃癌细胞具有浓度依赖性细胞毒作用。Hp抗菌肽 (2 2 0 )在相对较低浓度对胃黏膜上皮细胞有促进增殖的作用 ,可能与胃癌发生有关。
Objective To observe the in vitro effects of cecropin like anti bacterial peptide (2 20) from Helicobacter pylori ( H.pylori ) on gastric epithelial cells and gastric cancer cells. Methods The inhibition zone assay was used to determine anti bacterial activity and lethal concentrations of H.pylori anti bacterial peptide (2 20) and cecropin B on E.coli K12D31 (standard strain). The cytotoxicities and IC 50 of the H.pylori anti bacterial peptide (2 20) and cecropin B on human gastric epithelial cells and on gastric cancer cell lines were measured by MTT colorimatric methods. Results In vitro, the lethal concentrations (LC) of H.pylori anti bacterial peptide (2 20) and cecropin B on E.coli K12D31 were 65 μmol/L and 0.44 μmol respectively. The killing effects of cecropin B were more prominent than those of H.pylori anti bacterial peptide (2 20). The synthetic peptide H.pylori anti bacterial peptide(2 20) and cecropin B showed lytic or toxic activity against the human gastric cancer cell line SGC 7901 in a concentration dependent manner. The H.pylori anti bacterial peptide (2 20) stimulates the gastric epithelial cell GES 1 proliferation at the concentration ranging from 50 μmol/L to 200 μmol/L. The cecropin B has no lytic or toxic activety against gastric epithelial cell GES 1 at the concentration ranging from 5 μmol/L to 20 μmol/L. The IC 50 of H.pylori anti bacterial peptide (2 20)were 630 μmol/L for GES 1 cells and 500 μmol/L for SGC 7901 cells. The IC 50 of cecropin B were 75 μmol/L for GES 1 cells and 25 μmol/L for SGC 7901 cells respectively.Conclusions The H.pylori anti bacterial peptide (2 20) has a lower anti bacterial activity than cecropin B and there is a concentration dependent cytotoxic effect on the gastric cancer cells. The cecropin like antibacterial peptide from H.pylori anti bacterial peptide (2 20) stimulates the gastric epithelial cell proliferation at lower concentration and thus may be expected to increase the risk of gastric cancer.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2003年第12期715-717,共3页
Chinese Journal of Digestion
