摘要
以本实验室筛出的一株菌ST2 710为出发菌 ,研究不同培养条件对转化率的影响。结果发现 ,菌株ST2 710在种子培养基进行预培养 ,再转入转化培养基中培养 2d后 ,加入洛伐他汀溶液 1.0mL(10mg/mL)后再培养 5d ,产物的产率可达 4 3.4 1% ;就四种溶剂溶解底物条件进行了研究 ,结果发现用二甲基甲酰胺溶解洛伐他汀 ,产物产率为最高 ,并且转化作用不需要底物的诱导 ;
The optimal medium and the fermentation conditions of converting lovastatin by strain ST2710 were investigated. The results showed that strain ST2710 was preinoculated in seed media for two days, then 1mL of the seed culture was incubated into biotransformation media. After two days of growth, 1.0mL of calibration lovastatin was added and the incubation was continued for another five days. The productivity was 43.41%. The effects of different organic solvents on productivity were also investigated. And it was found the system of bioconversion required no induction with lovastatin; the rate of bioconversion with suspended cells was better than that with washed mycelium or the broth.
出处
《工业微生物》
CAS
CSCD
北大核心
2003年第4期17-19,共3页
Industrial Microbiology
关键词
微生物
转化条件
发酵条件
产率
lovastatin
bioconvertion
fermentation condition
