摘要
目的 探讨不明原因早期自然流产患者蜕膜巨噬细胞功能的变化。方法 以 2 5例早期自然流产患者为实验组 ,2 5例妊娠早期健康孕妇作为对照组 ,采用光镜及透射电镜观察、酶联免疫吸附实验、放射性同位素标记技术综合分析 ,对其蜕膜巨噬细胞百分率、超微结构、诱生TNF α及IL 1活性分别进行检测。结果 实验组与对照组相比较 ,巨噬细胞百分率未见显著变化 (P >0 .0 5 ) ,但超微结构显示功能活跃 ;实验组巨噬细胞TNF α、IL 1诱生活性上升 (均P <0 .0 0 0 5 )。结论 患者蜕膜巨噬细胞功能显著增高 ,可能参与了免疫学发病机制 。
Objective To analyse changes of decidual macrophage function from patients with unexplained spontaneous early abortion. Methods Twenty five healthy pregnancy women as control and twenty five patients enrolled for study.To observe the percentage of macrophage through microscope,ultrastructure through transmission electron microscope,the activity of tumor necrosis factor α and interleukin 1 through ELISA and 3 H TdR immuno labeling. Results The percentage of patient decidual macrophage has no significant change(P>0.05),but has the ultrastructure changes of active cell function;the activities of induced tumor necrosis factor α and interleukin 1 in patients increase greatly (all P<0.0005). Conclusion The function of patient decidual macrophage upregulates highly,these datas and the experimental evidences about the research of immunol pathogenesis,predict and prevent in this disease.
出处
《白求恩军医学院学报》
2003年第4期206-208,共3页
Journal of Bethune Military Medical College
关键词
自然流产
蜕膜
巨噬细胞
Spontaneous early abortion
Decidua
Macrophage
