摘要
目的 建立黄连上清片有效的质量控制方法。方法 采用薄层色谱法鉴别黄连、黄柏、大黄、白芷、栀子和黄芩 ;运用高效液相色谱法测定大黄素和大黄酚的含量。结果 薄层色谱方法简便、专属性强、重复性好 ;含量测定结果准确 ,大黄素与大黄酚平均回收率分别为 99.5 % (RSD =1.6 4 % ,n =5 )和 97.9% (RSD =1.32 % ,n =5 )。
OBJECTIVE: To establish the effective methods for the quality control of Huanglian Shangqing tablet. METHODS: The Rhizoma Coptidis and Cortex Phellodendri, Radix et Rhizoma Rhei, Radix Angelicae Dahuricae, Fructus Gardeniae, Radix Scutellariae were identified by TLC. The contents of emodin and chrysophanol were determined by HPLC. RESULTS: The TLC methods were simple with strong specificity and good reproducibility. The contents of emodin and chrysophanol were dertermined by HPLC which was accurate with the average recoveries of 99.5% (RSD =1.64%, n=5) and 97.9% (RSD=1.32%, n=5) respectively. CONCLUSION: These methods can be used to control the quality of Huanglian Shangqing tablet effectively.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第12期954-956,共3页
Chinese Pharmaceutical Journal
