摘要
目的 建立一个易于推广使用的新的血铅浓度检测方法。方法 全血样本经硝酸 高氯酸消化后 ,在pH 0 8～ 1 0条件下 ,使用氢化物发生原子荧光光谱仪测定铅含量。结果 血铅浓度在 0～ 6 0 0 μg/L范围内线性良好 ,r=0 999;测定高、中、低 3种浓度全血样本 ,批内变异系数 (CV)分别为 4 1%、3 6 %、3 5 % ,批间CV分别为 4 9%、4 6 %、4 7% ,回收试验的平均回收率分别为 10 5 4 %、98 5 %、94 8% ,对中国预防医学科学院系列浓度铅标准质控物质的单盲测定结果均在规定范围内 ;检出限为 0 3ng ;全血中主要二价阳离子对铅测定无明显干扰 ;样品在 4℃保存至少可稳定 1个月 ;使用该法测定天津市 86 9名儿童血铅含量 ,均值 91 9μg/L ,中位数 83 8μg/L ,范围 2 3 1～ 386 3μg/L ,呈正偏态分布。结论 该法对血铅测定是一个灵敏、准确、精密、廉价、快速的分析方法 ,适于我国绝大部分医疗机构开展铅防治工作的要求。
Objective To establish a new and convenient assay for whole blood lead concentration. Methods Whole blood sample was digested by nitric acid-perchloric. Under the condition of pH 0.8～1.0, lead concentration was examined by Hydridegeneration Atomic Fluorescence Spectrometry.Results The method had good linearity when lead concentration were between 0 to 600 μg/L, r =0.999. The within-run CV of the method for high, middle and low lead level were 4.1%, 3.6% and 3.5% respectively. The between-run CV were 4.9%, 4.6% and 4.7% respectively. The recovery rates were 105.4%, 98.5% and 94.8% respectively. The single-blind test result of series standard lead samples provided by Chinese Academy of Preventive Medicine were within the acceptable range. The detecting Limit of this method was 0.3 ng. There was no significant interference from main two-valence positiveion. The sample can be storage at lest for 1 months at 4℃. Samples of 869 children from Tianjin were tested by this method. The mean value was 91.9 μg/L, median was 83.8 μg/L, all data were within 23.1～386.3 μg/L. The result showed out positive skewness distribution.Conclusion This method were sensitive, accurate, precise, quick and low-cost. It was suitable for use in clinical laboratories.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2003年第11期677-679,共3页
Chinese Journal of Laboratory Medicine
