摘要
抗胃癌单克隆抗体MGb2对胃癌组织有较强的特异性,交联药物后对胃癌细胞株也表现出较强的体外杀伤效应。但抗体进入人体后能否定位于靶细胞上是实施临床治疗的关键。本文把标记上^(125)I的MGb2抗体在胃癌患者术前24小时静脉注射,术后采集胃癌组织固定后进行超薄切片,采用电镜下放射自显影的方法进行定位分析。结果表明,^(125)I—MGb2静脉注射后能特异地定位在靶细胞上并进一步内化入胞,但该抗体在肿瘤组织及细胞中的定位仍存在异质性。
Recently, remarkable advances have been made in the study and development of MGb2 against gastric cancer and immunoconjugates of MGb2 and anti-cancer agents, which have showed marked in vitro cytotoxic effect on target cells, have been successfully prepared. The localization on the target cells of the monoclonal antibody MGb2, in vivo, however is very important for the advanced clinical application. In the present study, 125I-MGb2 was injected into the vein of the patient with gastric cancer 24 hours before the operation, and after the operation, the specimens of gastric cancer were routinely fixed, embedded and smeared with HW4 nucleus emulsion. Electronic microscopy of ultra -thin sections autoradiographed showed that MGb2 was mainly localized on the cell membrane and microvilli, and could be internalized into the cytoplasm though the localization in the tumor tissue and tumor cells showed some heterogenecity.
出处
《临床肿瘤学杂志》
CAS
1996年第1期4-7,共4页
Chinese Clinical Oncology
关键词
胃癌
超微结构
抗体
克隆
肿瘤
Gastric cancer Monoclonal antibody, Electronic microscopy, Autoradiography
